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Human hyaluronidases map to a candidate tumor suppressor locus

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Abstract

Eukaryotic hyaluronidases are widely distributed, but until recently the only vertebrate enzyme to be cloned was the sperm-specific enzyme, PH20. We have now purified the hyaluronidase of human plasma. The identical enzyme, as well as a second proteolytically processed form, is present in urine. Amino acid sequence of the purified hyaluronidase matched a cDNA in the human Expressed Sequence Tag database which, in combination with 5′-RACE-PCR, was used to clone the gene, termed HYAL1, coding for a protein of 435 amino acids. HYAL1 is identical to an uncharacterized gene positionally cloned by others at chromosome 3p21.3 that is homozygously deleted in several small cell lung carcinoma cell lines. We have also identified two additional paralogous hyaluronidase-like genes flanking HYAL1 on chromosome 3p21.3 termed HYAL2 and HYAL3. We are evaluating the candidacy of these two genes as potential tumor suppressors. The mouse hyaluronidase gene, by convention termed Hyall, was also cloned and expressed and found to be 73% identical to the human enzyme. In mouse, serum hyaluronidase polymorphism has previously been mapped to 60 cM from the centromere of chromosome 9, which corresponds to a cytogenetic location of 9F1–F2, a region syntenic to the human 3p21.3. Mice with alleles of Hyall producing higher levels of plasma hyaluronidase have greater resistance to transplanted tumor growth. Products of these candidate tumor suppressor genes are being evaluated as potential anticancer agents.

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References

  1. Kreil G 1995Protein Sci. 4 1666

    CAS  Google Scholar 

  2. Frost G I, Csoka T and Stern R 1996Trends in Glycosci. Glycotechnol. 8 419

    CAS  Google Scholar 

  3. Afify A M, Stern M, Guntenhoner M and Stern R 1993Arch. Biochem. Biophys. 305 434

    Article  CAS  Google Scholar 

  4. Frost G I, Csóka T B, Wong T and Stern R 1997Biochem. Biophys. Res. Commun. 236 10

    Article  CAS  Google Scholar 

  5. Csóka T B, Frost G I, Wong T and Stern R 1997FEBS Lett. 417 307

    Article  Google Scholar 

  6. Csóka T B, Frost G I, Heng H H Q, Scherer S W, Mohapatra G and Stern R 1998Genomics 48 63

    Article  Google Scholar 

  7. De Maeyer E and De Maeyer-Guignard J 1992Int. J. Cancer 51 657

    Article  Google Scholar 

  8. Pawlowski A, Haberman H F and Menon I A 1979Int. J. Cancer 23 105

    Article  CAS  Google Scholar 

  9. Hosoe S, Shigedo Y, Ueno K, Tachibana I, Osaki T, Tanio Y, Kawase I, Yamakawa K, Nakamura Y and Kishimoto T 1994Lung Cancer 10 297

    Article  CAS  Google Scholar 

  10. Mitsudomi T, Oyama T, Nishida K, Ogami A, Osaki T, Sugio K, Yasumoto K, Sugimachi K and Gazdar A 1996Clin. Cancer Res. 2 1185

    CAS  Google Scholar 

  11. Todd S, Franklin W A, Varella-Garcia M, Kennedy T, Hilliker C E, Jr, Hahner L, Anderson M, Wiest J S, Drabkin H A and Gemmill R M 1997Cancer Res. 57 1344

    CAS  Google Scholar 

  12. Kovatich A, Friedland D M, Druck T, Hadaczek P, Huebner K, Comis R L, Hauck W and McCue P A 1998Cancer 83 1109

    Article  CAS  Google Scholar 

  13. Weigel P H, Hascall V C and Tammi M 1997J. Biol. Chem. 272 13997

    Article  CAS  Google Scholar 

  14. Prehm P 1984Biochem. J. 220 597

    CAS  Google Scholar 

  15. Toole B P 1991 InCell biology of the extracellular matrix (ed.) E Hay (New York: Plenum), pp 305–341

    Google Scholar 

  16. Laurent T C and Fraser J R 1992FASEB J. 6 2397

    CAS  Google Scholar 

  17. Turley E A 1992Cancer Metastasis Rev. 11 21

    Article  CAS  Google Scholar 

  18. Underhill C B 1992J. Cell Sci. 103 293

    CAS  Google Scholar 

  19. Naor D, Sionov R V and Ish-Shalom D 1997Adv. Cancer Res. 71 241

    Article  CAS  Google Scholar 

  20. Lesley J and Hyman R 1998Front Biosci. 3 616

    Google Scholar 

  21. Hall C L and Turley E A 1995J. Neurooncol. 26 221

    Article  CAS  Google Scholar 

  22. Entwistle J, Hall C L and Turley E A 1996J. Cell Biochem. 61 569

    Article  CAS  Google Scholar 

  23. Toole B P 1990Curr. Opin. Chem. Biol. 2 839

    CAS  Google Scholar 

  24. Knudson C B and Knudson W 1993FASEB J. 7 1233

    CAS  Google Scholar 

  25. Wight T, Heingard D and Hascall V 1991 InCell biology of extracellular matrix. (ed.) E Hay (New York: Plenum Press) pp. 45–78

    Google Scholar 

  26. Zhao M, Yoneda M, Ohashi Y, Kurono S, Iwata H, Ohnuki Y and Kimata K 1995J. Biol. Chem. 270 26657

    Article  CAS  Google Scholar 

  27. Knudson W 1996Am. J. Pathol. 148 1721

    CAS  Google Scholar 

  28. Zhang L, Underhill C B and Chen L 1995Cancer Res. 55 428

    CAS  Google Scholar 

  29. De Salegui M and Pigman W 1967Arch. Biochem. Biophys. 120 60

    Article  Google Scholar 

  30. Fenger M 1982J. Chromatogr. 240 173

    Article  CAS  Google Scholar 

  31. Zhu L, Hope T J, Hall J, Davies A, Stern M, Muller-Ebernard U, Stern R and Parslow T G 1994J. Biol. Chem. 269 32092

    CAS  Google Scholar 

  32. Hrkal Z, Kuzelová K, Muller-Eberhard U and Stern R 1996FEBS Lett. 383 72

    Article  CAS  Google Scholar 

  33. Nadeau J H 1986Genet. Res. 48 175

    Article  CAS  Google Scholar 

  34. Imai K 1997Mammalian Genome 7 S159

  35. Lepperdinger G, Strobl B and Kreil G 1998J. Biol. Chem. 273 22466

    Article  CAS  Google Scholar 

  36. Berlyne G M 1960Clin. Sci. 19 619

    CAS  Google Scholar 

  37. Cobbin L B and Dicker S E 1962J. Physiol. 163 168

    CAS  Google Scholar 

  38. Dicker S E and Franklin C S 1966J. Physiol. 186 110

    CAS  Google Scholar 

  39. Fiszer-Szafarz B 1984Anal. Biochem. 143 76

    Article  CAS  Google Scholar 

  40. Cherr G N, Meyers S A, Yudin A I, VandeVoort C A, Myles D G, Primakoff P and Overstreet J W 1996Dev. Biol. 175 142

    Article  CAS  Google Scholar 

  41. Hunnicutt G R, Mahan K, Lathrop W F, Ramarao C S, Myles D G and Primakoff P 1996Biol. Reprod. 54 1343

    Article  CAS  Google Scholar 

  42. Renkema G H, Boot R G, Strijland A, Donker-Koopman W E, van den Berg M, Muijsers A O and Aerts J M 1997Euro. J. Biochem. 244 279

    Article  CAS  Google Scholar 

  43. Fiszer-Szafarz B and De Maeyer E 1989Somatic Cell Mol. Genet. 15 79

    Article  CAS  Google Scholar 

  44. De Maeyer-Guignard J, Cachard-Thomas A and De Maeyer E 1991J. Exp. Zool. 258 246

    Article  Google Scholar 

  45. McBride W H and Bard J B 1979J. Exp. Med. 149 507

    Article  CAS  Google Scholar 

  46. Dick S J, Macchi B, Papazoglou S, Oldfield E H, Kornblith P L, Smith B H and Gately M K 1983Science 220 739

    Article  CAS  Google Scholar 

  47. Knudson W, Biswas C, Li X Q, Nemec R E and Toole B P 1989Ciba Found. Symp. 413 150; discussion pp 159–69, 281–5

    Google Scholar 

  48. Ozzello L, Lasfargues E and Murray M 1960Cancer Res. 20 600

    CAS  Google Scholar 

  49. Toole B P, Biswas C and Gross J 1979Proc. Natl. Acad. Sci. USA 76 6299

    Article  CAS  Google Scholar 

  50. Fiszer-Szafarz B and Gullino P M 1970Proc. Soc. Exp. Biol. Med. 133 805

    CAS  Google Scholar 

  51. Fiszer-Szafarz B and Szafarz D 1973Cancer Res. 33 1104

    CAS  Google Scholar 

  52. Wilkinson C R, Bower L M and Warren C 1996Clin. Chim. Acta 256 165

    Article  CAS  Google Scholar 

  53. Wei M H, Latif F, Bader S, Kashuba V, Chen J Y, Duh F M, Sekido Y, Lee C C, Geil L, Kuzmin I, Zabarovsky E, Klein G, Zbar B, Minna J D and Lerman M I 1986Cancer Res. 56 1487

    Google Scholar 

  54. Hibi K, Takahashi T, Yamakawa K, Ueda R, Sekido Y, Ariyoshi Y, Suyama M, Takagi H and Nakamura Y 1992Oncogene 7 445

    CAS  Google Scholar 

  55. Wu C L, Sloan P, Read A P, Harris R and Thakker N 1994Cancer Res. 54 6484

    CAS  Google Scholar 

  56. Hung J, Kishimoto Y, Sugio K, Virmani A, McIntire D D, Minna J D and Gazdar A F 1995J. Am. Med. Assoc. 273 1908

    Article  CAS  Google Scholar 

  57. Yokoyama S, Yamakawa K, Tsuchiya E, Murata M, Sakiyama S and Nakamura Y 1992Cancer Res. 52 873

    CAS  Google Scholar 

  58. Daly M C, Xiang R H, Buchhagen D, Hensel C H, Garcia D K, Killary A M, Minna J D and Naylor S L 1993Oncogene 8 1721

    CAS  Google Scholar 

  59. Lu Y Y, Jhanwar S C, Cheng J Q and Testa J R 1994Genes, Chromosomes Cancer 9 76

    Article  CAS  Google Scholar 

  60. Craighead J E and Mossman B T 1982N. Engl. J. Med. 306 1446

    CAS  Google Scholar 

  61. Cheng Y, Poulos N, Lung M, Hampton G, Ou B, Lerman M and Stanbridge E 1998Proc. Natl. Acad. Sci. USA 95 3042

    Article  CAS  Google Scholar 

  62. Huang D P, Ho J H, Chan W K, Lau W H and Lui M 1989Int. J. Cancer 43 936

    Article  CAS  Google Scholar 

  63. Latif F, Duh F M, Bader S, Sekido Y, Li H, Geil L, Zbar B, Minna J D and Lerman M I 1997Hum. Genet. 99 334

    Article  CAS  Google Scholar 

  64. Sekido Y, Ahmadian M, Wistuba II, Latif F, Bader S, Wei M H, Duh F M, Gazdar A F, Lerman M I and Minna J D 1998Oncogene 16 3151

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Gerontology, University Medical School of Debrecen, Hungary

    Tony Benjamin Csóka

  2. Department of Pathology, University of California, San Francisco, USA

    Robert Stern

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  1. Tony Benjamin Csóka
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  2. Robert Stern
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Correspondence to Robert Stern.

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Csóka, T.B., Stern, R. Human hyaluronidases map to a candidate tumor suppressor locus. Proc. Indian Acad. Sci. (Chem. Sci.) 111, 275–281 (1999). https://doi.org/10.1007/BF02869916

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