Influence of NaCl, KCl and MgSO4 concentration on total and irreversible adsorption of T2r phage on isolated cell walls
- 92 Downloads
The dependence on salt concentration of steady-state total and irreversible adsorption of T2r phage on isolated bacterial cell walls was studied. Solutions of NaCl, KCl and MgSO4 of different molarity were used and a difference between the total and the irreversible adsorption at lower concentration of NaCl and KCl, but not in MgSO4 solutions, was found. The effect of KCl on total adsorption was similar to that of MgSO4 in which case the adsorption begins at lower molar concentrations than with NaCl.
KeywordsBacterial Cell Wall Irreversible Adsorption Total Adsorption Reversible Adsorption Phage Titration
Unable to display preview. Download preview PDF.
- Černohorský, I. J., Vondrejs, V., Závada, V.:Purification and concentration of f 2 and T2 phage lysate on calcium phosphate column. Fol. microbiol. 13: 250, 1968.Google Scholar
- Garen, A., Kozloff, L. M.:The initiation of bacteriophage infection. The Viruses, eds. Burnet, F. M., and Stanley, W. M., Academic Press, New York-London, p. 203, 1959.Google Scholar
- Puck, T. T.:The first steps of virus invasion. Cold Spring Harbor Symposia Quant. Biol. 18: 149, 1953.Google Scholar
- Salton, M. R. J.:Isolation of bacterial cell walls. The bacterial cell wall, Elsevier Publ. Company, Amsterdam, pp. 42–63, 1964.Google Scholar
- Zárybnický, V., Horáček, P.:Influence of ionic strength on the stability of phage T2r to osmotic shock. Fol. microbiol. 13: 391, 1968a.Google Scholar
- Zárybnický, V., Horáček, P.:Der innere Druck im Kopfe des Bakteriophagen T2r. Studia biophysica 8: 179, 1968b.Google Scholar