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Relationship between the carbohydrate metabolism ofStreptomyces aureofaciens and the biosynthesis of chlortetracycline

I. The effect of interrupted aeration, inorganic phosphate and benzyl thiocyanate on chlortetracycline biosynthesis

Соотношение между углеводным оБменом у Streptomyces Aureofaciens и Биосин тезом хлортетрациклина

I. Влуцянце перерывов в аэраццц, ормофосфама ц роданцсмоео бензцла на бцосцнмез шлормемраццклцна

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Abstract

The effect of interrupted aeration on the biosynthesis of chlortetracycline (CTC) was investigated. The culture is most sensitive to interruption in aeration when between the 6th and 12th hour of growth. Then even short interruptions will result in a pronounced suppression of CTC biosynthesis. Using glucose labelled at carbon 1 and at carbon 6 with14C it could be demonstrated that the interruption in aeration brings about a decrease in the activity of the pentose shunt during breakdown of sugar in the course of subsequent cultivation. A similar effect can be induced by increasing the level of inorganic phosphate in the medium. It was shown by studying the interaction of benzyl thiocyanate and interruption of aeration on the biosynthesis of CTC that benzyl thiocyanate antagonizes the unfavourable effect of interrupted aeration. Its presence will prevent a drop in CTC production by a culture aerated with interruptions. The relationship between the enzymatic reactions of the pentose shunt and the mechanism of chlortetracycline biosynthesis is discussed.

Abstract

Изучалось влияние перерывов в аэрации на биосинтез хлортетрациклина (CTC). Культура наиболее чувствительна к перерывам в аэрации между 6-м и 12 час. выращивания, когда короткие перерывы аэрации резко угнетают биосинтез CTC. С помощью глюкозы, специфически меченой14C на первом или на шестом углероде, было доказано, что перерывы в аэрации понижают активность пентозового цикла при диссимиляции сахара в ходе дальнейшего культивирования. Такое же действие, как приостановка аэрации, в чувствительной фазе оказывает и повышение содержания ортофосфата в культивационной среде. При изучении совместного действия роданистого бензила и перерывов в азрации на биосинтес CTC было доказано, что роданистый бензил является антагонистом неблагоприятного действия перерывов в аэрации. Присутствие роданистого бензила предупреждает понижение продукции CTC при интервалах в аэрации.—Обсуждается связь связь между энзиматическими реакциями пентозового цикла и механизмом биосинтеза хлортетрациклина.

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  1. Research Institute of Antibiotics, Roztoky near Prague

    Z. Hošťálek

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Hošťálek, Z. Relationship between the carbohydrate metabolism ofStreptomyces aureofaciens and the biosynthesis of chlortetracycline. Folia Microbiol 9, 78–88 (1964). https://doi.org/10.1007/BF02868788

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