Abstract
Depending on conditions of aeration maltose and glucose were found to exhibit different effects on the inducible synthesis of β-galactosidase in aerobically grown cells ofEscherichia coli starving for an exogenous source of nitrogen; both saccharides repressed the synthesis of the enzyme under aerobic conditions, while the above-mentioned saccharides were essential for the enzyme synthesis under anaerobic conditions. The presence of maltose in the medium resulted in the repression of the enzyme synthesis in anaerobically grown cells starving for an exogenous nitrogen source under anaerobic conditions. The synthesis of β-galactosidase-specific messenger RNA was completely blocked and the synthesis of the enzyme proper considerably inhibited in aerobically grown cells incubated anaerobically in a medium without nitrogen and carbon sources.
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Cohn, M., Horibata, K.:Physiology of the inhibition by glucose of the induced synthesis of β-galactosidase-enzyme system of Escherichia coli. J. Bacteriol. 78: 624, 1954.
Černý, M., Pacák, J.:A new method to prepare acetylated α-D-glucopyranosides. (In Czech.) Chem. Listy 52: 2090, 1958.
Dawes, E. A., Ribbons, D. W.:Studies on the endogenous metabolism of Escherichia coli. Biochem. J. 95: 332, 1965.
Dobrogosz, W.:The influence of nitrate and nitrite reduction on catabolite repression in Escherichia coli. Biochim. biophys. Acta 100: 553, 1965.
Faith, W. T., Giorgio, N. A., Mallette, M. F.:Mechanism of glucose inhibition of β-galactosidase biosynthesis in resting cultures of Escherichia coli. Arch. Biochem. Biophys. 108: 430, 1964.
Fowler, C. B.:The relationship between fermentation and enzymatic adaptation. Biochim. biophys. Acta 7: 563, 1951.
Kováč, L., Kužela, Š.:Effect of uncoupling agents and azide on the synthesis of β-galactosidase in aerobically and anaerobically grown Escherichia coli. Biochim. biophys. Acta 127: 355, 1966.
Loomis, W. F., Magasanik, B.:Nature of the effector of catabolite repression of β-galactosidase in Escherichia coli. J. Bacteriol. 92: 170, 1966.
Magasanik, B.:Catabolite repression. Cold Spring Harbor Symp. Quant. Biol. 26: 249, 1961.
Mandelstam, J.:The intracellular turnover of proteins and nucleic acids and its role in biochemical differentiation. Bacteriol. Rev. 24: 289, 1960.
Mandelstam, J.:Induction and repression of β-galactosidase in non-growing Escherichia coli. Biochem. J. 79: 489, 1961.
McFall, E., Mandelstam, J.:Specific metabolic repression of three induced enzymes in Escherichia coli. Biochem. J. 89: 391, 1963.
Moses, V., Prevost, C.:Catabolite repression of β-galactosidase synthesis in E. coli. Biochem. J. 100: 336, 1966.
Nakada, D., Magasanik, B.:The roles of inducer and catabolite repressor in the synthesis of β-galactosidase by Escherichia coli. J. mol. Biol. 8: 105, 1964.
Pardee, A. B., Prestidge, L. S.:The initial kinetics of enzyme induction. Biochim. biophys. Acta 49: 77, 1961.
Seidman, M., Link, K. P.:O-nitrophenyl-β-D-galactoside and its tetraacetate. J. Am. Chem. Soc. 72: 4324, 1950.
Szulmajster, J., Grunberg-Manago, M., Prouvost, A.:Sur le métabolisme aérobie et anaérobie de Escherichia coli. Biochim. biophys. Acta 9: 636, 1952.
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Kužela, Š., Kováč, L. The effect of anaerobiosis on the induced synthesis of β-galactosidase in non-growingEscherichia coli . Folia Microbiol 13, 129–133 (1968). https://doi.org/10.1007/BF02868213
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DOI: https://doi.org/10.1007/BF02868213