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Indian Journal of Clinical Biochemistry

, Volume 12, Issue 1, pp 86–90 | Cite as

Modulation of convulsive threshold of pentylene tetrazole by zinc

  • N. Khanna
  • A. Garg
  • K. K. Sharma
  • R. Khosla
Minerals

Abstract

In the present study which was aimed to see the effect of zinc on pentylenetetrazole induced convulsive threshold in rats we found that zinc sulfate 100 μg intracerebroventricularly) and pentylenetetrazole (50 mg/kg intraperitoneally) produced dose related seizure activity; however, pretreatment with zinc decreased the threshold, increased the severity, incidence of multiple seizures and total duration of pentylenetetrazole induced seizures. Diazepam (5 mg/kg intraperitoneally) pretreatment increased the threshold and lowered the incidence of convulsions in zinc induced seizures which further confirms that the convulsive effect of zinc is through inhibition of gamma aimino butyric acid.

Key Words

Zinc Pentylentetrazole Diazepam GABA 

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References

  1. 1.
    Purpura, D.P., Penry, J.K., Tower, D.B., Woodbury, D.M. and Walter, R.D. (1972) Experimental models of epilepsy. Raven Press, New York.Google Scholar
  2. 2.
    Noble, E.P., Wurtman, R.T. and Axelrod, J. (1967) A simple and rapid method for injection of H3-norepinephrine into the lateral ventricle of the rat brain. Life Sciences. 6, 281–291.PubMedCrossRefGoogle Scholar
  3. 3.
    Pei, Y.Q. and Koyama, I. (1986) Features of seizures and behavioural changes induced by intrahippocampal injection of zinc sulfate in the rabbit: a new experimental model of epilepsy. Epilepsia. 27(3), 183–188.PubMedCrossRefGoogle Scholar
  4. 4.
    Rosen, A.D. and Frumin, N.V. (1979) Focal epileptogenesis after intracortical haemoglobin injection. Experimental Neurology, 66, 277–284.PubMedCrossRefGoogle Scholar
  5. 5.
    Reid, S.A., Sypert, G.W. and Goggs, W.M. (1979) Histopathology of the ferri-induced chronic epileptic focus in cat. Exp. Neurol. 66, 205–210.PubMedCrossRefGoogle Scholar
  6. 6.
    Lange, S.C., Nealsey, E.J. and Wyler, A.R. (1980) Neuronal activity in chronic ferric chloride epileptic foci in cats and monkeys. Epilepsia. 21, 251–254.PubMedCrossRefGoogle Scholar
  7. 7.
    Willmore, I.J. (1978) Recurrent seizure induced by cortical iron injection, a model of post-traumatic epilepsy. Ann. Neurol. 4, 329–333.PubMedCrossRefGoogle Scholar
  8. 8.
    Pei, Y. and Xie, S. (1980) A new experimental model of epilepsy. Yao Hsuch Hsuch Pao. 15, 113–117.Google Scholar
  9. 9.
    Tokuoka, S. (1967) Neurochemical consideration on the alleviating effect of caudal resection of the pancreas on epileptic seizure relationship of zinc metabolism to brain excitability. Bull Yamaguchi Med. Sch. 14, 1–18.Google Scholar
  10. 10.
    Alley, N.C., Killam, E.K. and Fisher, G.I. (1981) The influence of D-penicillamine treatment upon seizure activity and trace metal state in the Senegalese baboon, Papio papio. J. Pharmacol. Exp. Ther. 217, 138–146.PubMedGoogle Scholar
  11. 11.
    Papavasiion, P.S., Kutt, M., Miller, S.T., Rosal, V., Wang, Y.Y. and Aronson, R.B. (1979) Seizure disorders and trace metal, manganese tissue levels in treated epileptics. Neurology. 29, 1466–1479.Google Scholar
  12. 12.
    Pei, Y.Q., Zhao, D., Haung, J. and Cao, L. (1983) Zinc-induced seizures: a new experimental model of epilepsy. Epilepsia. 24, 169–176.PubMedCrossRefGoogle Scholar
  13. 13.
    Mares, P. and Schickerova, R. (1980) Seizures elicited by subcutaneous injection of Metrazole during ontogenesis in rats. Act. Nerv. Super. 22, 264–268.Google Scholar
  14. 14.
    Lacoste, L. and Bartolucci, S. (1987) Pentylenetetrazole inhibits glutamate dehydrogenase and aspartate aminotransferase, and stimulates GABA aminotransferase in homogenates from rat cerebral cortex. Can. J. Physiol. Pharmacol. 66, 1135–1138.Google Scholar

Copyright information

© Association of Clinical Biochemists of India 1997

Authors and Affiliations

  • N. Khanna
    • 1
  • A. Garg
    • 1
  • K. K. Sharma
    • 1
  • R. Khosla
    • 1
  1. 1.Department of PharmacologyUniversity College of Medical Sciences & G.T.B. HospitalDelhi

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