Indian Journal of Clinical Biochemistry

, Volume 18, Issue 1, pp 71–74 | Cite as

Lipid peroxidation in erythrocytes of patients with type 2 diabetes mellitus

  • P. Ashok Kumar
  • G. Rajagopal


Lipid peroxidation by measurement of Thiobarbituric Acid Reactive Substances (TBARS) and antioxidant status by determining the activities of the enzymes, Catalase (Cat), Glutathione Peroxidase (GPx) and Superoxide Dismutase (SOD) and the level of Reduced Glutathione (GSH) in the erythrocytes of patients with type 2 Diabetes Mellitus were investigated. It was observed that the level of TBARS in the erythrocytes was increased by 50% showing a significant generation of free radicals in the erythrocytes of these patients. The activities of both Cat and SOD were enhanced while that of GPx was not altered. The level of GSH was also not changed.

Key words

Glutathione Type 2 Diabetes Catalase Peroxidase Superoxide Dismutase 


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  1. 1.
    Murray, K, Daryl K. Garner, Peter A Mayer, Victor N Rodwell in Harper's Biochemistry 25th edn Lange 2000.Google Scholar
  2. 2.
    Lakshmi, S and Rajagopal, G (1998) Reduced Glutathione level and Catalase activity in erythrocytes in patients with Diabetes Mellitus. Biomedicine. 18(1) 37–39.Google Scholar
  3. 3.
    Drabkin, DL, and Austin, JM, (1932) Spectrometric studies and spectrometric constants for common haemoglobin derivatives in human, dog and rabbit blood. J.Biol.Chem.Google Scholar
  4. 4.
    Buetler and Kelly, (1963) The effect of sodium on RBC Glutathione. J.Experientia 19:96.CrossRefGoogle Scholar
  5. 5.
    Rotruck, JT, Pope, L Ganther, HE, Swanson, AB. (1973) Selenium biochemical role as a component of Glutathione Peroxidase. Science. 179:588.PubMedCrossRefGoogle Scholar
  6. 6.
    Sinha, K.A. (1972) Colorimetric assay of catalase. J. Biochem. 47: 389.Google Scholar
  7. 7.
    Trinder, P. (1969). Annals clin. Chem 6:24Google Scholar
  8. 8.
    Ohkawa, H Ohisi, N and Yagi, K. (1979). Assay of lipid peroxides in animal tissues by thiobarbituric reaction. Annal. Biochem. 95:351.CrossRefGoogle Scholar
  9. 9.
    Kakkar, P, Das, B and Viswanathan, PN, (1984) Indian J. of Biochemistry and Biophysics 21:130.Google Scholar
  10. 10.
    Parthiban, A, Vijayalingam, S, Shanmugasundaram, KR, Mohan, S, (1995) Oxidative stress and development of diabetic complications-antioxidants and lipid peroxidation in erythrocytes and cell membrane. Cell-Biol-Int. Dec: 19(12):987–910CrossRefGoogle Scholar
  11. 11.
    Oberley, LW, (1988) Free radicals and diabetes. Free radical. Biol. ed., 5:113.CrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2003

Authors and Affiliations

  • P. Ashok Kumar
    • 1
  • G. Rajagopal
    • 1
  1. 1.Department of Biochemistry, Rajah Muthiah Medical CollegeAnnamalai UniversityAnnamalai Nagar

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