Folia Microbiologica

, Volume 15, Issue 1, pp 23–33 | Cite as

Study of the reciprocal relationship of the exoantigens ofClostridium perfringens type A in the culture medium and the bacterial cells during cultivation

  • G. F. Shemanova
  • V. I. Gorshkova
  • O. K. Borisova
  • K. L. Shakhanina


The times of the active release ofClostridium perfringens antigens—lecithinase, collagenase and alkaline proteinase—were determined. It was found that lecithinase release attained maximum values after 4–9 hours' cultivation of the microorganisms and stopped completely after 24–36 hours. The course of collagenase release was the same. Maximum proteinase release was determined within 36 to 48 hours' cultivation ofClostridium perfringens. Preformed lecithinase disappeared very rapidly from the culture medium. The presence of the components of the exoantigen complex was determined in the bacterial cell cytoplasm by the immunoadsorption method. Their distribution in the fractions isolated by differential high speed centrifugation of homogenates of disintegrated bacterial cells was studied. Their ribosomal fraction, represented by four groups of ribosomes with the maximum sedimentation coefficients 110 and 130S, contains alkaline proteinase. Its activity diminishes during cultivation of the microorganism and proteolytic activity accumulates in the hyaloplasm. The absence of lecithinase and collagenase in the bacterial cells was demonstrated.


Alkaline Proteinase Active Release Clostridium Perfringens Toxigenic Strain Bacterial Mass 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1970

Authors and Affiliations

  • G. F. Shemanova
    • 1
  • V. I. Gorshkova
    • 1
  • O. K. Borisova
    • 1
  • K. L. Shakhanina
    • 1
  1. 1.Institute of Medico-biological Problems, Ministry of Health of the U.S.S.R. and Gamaleya Institute of Epidemiology and MicrobiologyAcademy of Medical Sciences of the U.S.S.R.Moscow

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