Study of systemic fever reaction of the delayed type hypersensitivity
- 25 Downloads
The possibility of elaborating a model of fever reaction to a simple protein antigen, ovalbumin, was investigated. Administration of the antigen in adjuvants into the foot-pads or intravenously proved unsatisfactory and did not sensitize the animal to induction of a fever reaction to a challenging dose of antigen. Sensitization by the simultaneous intravenous administration of ovalbumin together with living BCG vaccine yielded positive results. The fever response to ovalbumin is specific, since rabbits sensitized with BCG vaccine only did not respond to the administration of ovalbumin. The degree of fever reactions to tuberculin and ovalbumin in the individual rabbits was more or less proportional. The given model is reproducible and is useful for experimental studies. Further experiments will be necessary, however, for detailed characterization and for analysis of the mechanism (antibody-mediated or delayed type hypersensitivity).
KeywordsIntravenous Administration Rectal Temperature Protein Antigen Delay Type Hypersensitivity Febrile Response
Unable to display preview. Download preview PDF.
- Castrová, A., Pekárek, J., Johanovský, J., Švejcar, J., Malý, V.:Study of systemic fever reaction of the delayed type hypersensitivity. II. Influence of the site of administration of the sensitizing antigen and the time of sensitization on the intensity of the systemic fever reaction. Z. Immun. Forsch. 130: 263, 1966a.Google Scholar
- Castrová, A., Pekárek, J., Johanovský, J., Švejcar, J.:Study on systemic reaction of delayed type hypersensitivity. I. Various effects of two different samples of PPD tuberculins on systemic reaction and other manifestations of delayed type hypersensitivity. Fol. microbiol. 11: 123, 1966b.Google Scholar
- Eisen, H. N.:Hypersensitivity to simple chemicals. Cellular and humoral aspects of the hypersensitive states. Ed. H. S. Lawrence, London, p. 89, 1959.Google Scholar
- Farr, R. S.:The febrile response upon injection of bovine albumine into previously sensitized rabbits. J. clin. Invest. 37: 894, 1958.Google Scholar
- Kolín, A., Johanovský, J., Pekárek, J.:Histological manifestations of cellular (delayed) hypersensitivity. II. Regressive changes of the subcutaneous musculature in the skin test to ovalbumin in guinea-pigs. Intern. Arch. Allergy 26: 167, 1965.Google Scholar