Folia Microbiologica

, Volume 12, Issue 5, pp 441–446 | Cite as

Growth ofEscherichia coli B in a continuous culture under limitation by inorganic phosphate

  • J. Slezák
  • B. Sikyta


During batch cultivation ofEscherichia coli in a medium deficient in inorganic phosphate, the growth curve after exhaustion of phosphate is linear. Results obtained in batch cultivation were used for deriving expressions for bacterial growth at a constant rate in a single-and multi-stage continuous system. It was found experimentally that the theoretical relations derived are satisfactory.


Specific Growth Rate Batch Cultivation Dilution Rate Linear Phase Constant Growth Rate 
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  1. Fiske, C. H., Subbarow, Y.:The colorimetric determination of phosphorus. J. biol. Chem. 66: 375, 1925.Google Scholar
  2. Herbert, D.:Some principles of continuous culture. Recent Progress in Microbiology, Almquist and Wiksell Stockholm p. 381, 1958.Google Scholar
  3. Holme, T.:Continuous culture studies on glycogen synthesis in Escherichia coli B. Acta chem. Scand. 11: 763, 1957.CrossRefGoogle Scholar
  4. Horiuchi, T.:RNA degradation and DNA and protein synthesis of E. coli B in a phosphate deficient medium. J. Biochem. 46: 1467, 1959.Google Scholar
  5. Jackson, F. L., Copping, A. M.:Some aspects of the growth of Proteus vulgaris with limiting concentrations of nicotinic acid. J. gen. Microbiol. 7: 107, 1952.PubMedGoogle Scholar
  6. Karush, F., Iacocca, V. I., Harris, T. N.:Growth of group a hemolytic streptocous in the steady state. J. Bacteriol. 72: 283, 1956.PubMedGoogle Scholar
  7. Luedeking, R., Piret, E. L.:Transient and steady state in continuous fermentation. Theory and experiment. J. Biochem. Micr. Technol. Eng. 1: 431, 1959.CrossRefGoogle Scholar
  8. Monod, J.:La technique de culture continue; thérie et applications. Ann. Inst. Pasteur 79: 390, 1950.Google Scholar
  9. Novick, A., Szilard, L.:Description of the chemostat. Science 112: 715, 1950.PubMedCrossRefGoogle Scholar
  10. Postgate, J. R., Hunter, J. R.:The survival of starved bacteria. J. gen. Microbiol. 29: 233, 1962.PubMedGoogle Scholar
  11. Řičica, J., Hospodka, J.:Laboratory apparatus for batch and continuous flow cultivation of microorganisms. (In Czech). Kvasný průmysl 6: 175, 1960.Google Scholar
  12. Schaeffer, P.:Croissance et respiration d’une souche streptomycino-exigeante de Bacillus cereus privée de l’antibiotique—facteur de croissance. Ann. Inst. Pasteur 78: 624, 1950.Google Scholar
  13. Tempest, D. W., Hunter, J. R., Sykes, J.:Magnesium—limited growth of Aerobacter aerogenes in a chemostat. J. gen. Microbiol. 39: 355, 1965.PubMedGoogle Scholar
  14. Ulbert, S.:Study of the logarithmic and linear growth of Escherichia coli B during limitation by inorganic phosphate. (In Czech.) Thesis, College of Chemical Technology, Prague 1964.Google Scholar
  15. Yerusalimsky, N. D., Neronova, N. M.:Quantitative dependence between the concentration of metabolic products and the rate of growth of microorganisms. (In Russian). Dokl. Akad. Nauk SSSR 161: 1437, 1965.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1967

Authors and Affiliations

  • J. Slezák
    • 1
  • B. Sikyta
    • 1
  1. 1.Research Institute of Antibiotics and BiotransformationRoztoky near Prague

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