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Chinese Journal of Oceanology and Limnology

, Volume 19, Issue 2, pp 135–140 | Cite as

Up-regulation of hepatic receptor for growth hormone in the flounder (Paralichthys olivaceus) after oral administration with exogenous GH

  • Liu Zong-zhu 
  • Wang Jin-bao 
  • Xu Yong-li 
  • Wang Yong 
  • Zhang Pei-jun 
Biology
  • 33 Downloads

Abstract

The iodination efficiency of salmon GH(sGH) was 38.82%, using a modification of the chloramine-T method. The specific activity of the125I-sGH was about 40 μCi/μg protein. The results of binding assay showed a single class of high affinity and low-capacity binding site in flounder liver. Long-term administration with exogenous GH can induce the up-regulation of hepatic GH receptor in total binding capacity though there was no significant difference in capacity of free binding sites of livers from control and experimental fish, this result also indicated that the liver from experimental fish, compared to that from control fish, had more occupied binding sites.

Key words

iodination GH hepatic receptor up-regulation 

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Copyright information

© Science Press 2001

Authors and Affiliations

  • Liu Zong-zhu 
    • 1
    • 2
  • Wang Jin-bao 
    • 2
  • Xu Yong-li 
    • 1
  • Wang Yong 
    • 1
  • Zhang Pei-jun 
    • 1
  1. 1.Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Dep. Anim. Sci. Laiyang Agriculture CollegeLaiyang

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