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Quantitative determination of tyrosinase activity using paper discs and a recording densitometer

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Summary

A paper-disc technique was used for the quantitative determination of tyrosinase activity. By measuring with a recording densitometer the color which developed 20 min after application of the enzyme in a water-saturated atmosphere in a plastic cylinder tyrosinase activity was determined.

Resumen

Un método de disco de papel ha sido usado para determinar cuantitativamente la actividad de tirosinasa. Ha sido possible medir la cantidad de actividad de tirosinasa midiendo con un densímetro grabador el color que apareció 20 min después de aplicar la enzima en una atmósfera saturada de agua en un cilindro plástico.

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Author notes

  1. M. L. Weaver (Assistant Professor of Horticulture)

    Present address: Western Utilization Research and Development Division, 94710, Albany, California

Authors and Affiliations

  1. Washington State University, Pullman

    M. L. Weaver (Assistant Professor of Horticulture)

  2. Department of Hortculture, Washington State University, Pullman

    H. A. Steen (Laboratory Assistant)

Authors
  1. M. L. Weaver
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  2. H. A. Steen
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Additional information

Scientific Paper 2780, College of Agriculture, Washington State University. Work was conducted under project 1480.

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Weaver, M.L., Steen, H.A. Quantitative determination of tyrosinase activity using paper discs and a recording densitometer. American Potato Journal 44, 387–392 (1967). https://doi.org/10.1007/BF02862533

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  • DOI: https://doi.org/10.1007/BF02862533

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