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Use of molecular gel column to prepare potato virus X inocula for antisera

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Summary

A simple technique has been developed to obtain purified potato virus X in physiological saline solution by passage through a gel column. The virus solution is the concentrated by dialysis with polyethylene glycol for use in intravenous injections for the production of potato virus X-immune rabbit antiserum.

Resumen

Se ha desarrollado una técnica sencilla para obtener el virus X de patata purificado en una solución salina fisiólógica por pasarse por una columna de gelatina. Luego, se concentra la solución del virus por diálisis con el glicol polietileno para usarse en intervenciones in travenosas para la producción del antisuero de conejo inmune del virus X de patata.

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Literature Cited

  1. Bawden, F. C. and A. Kleczkowski. 1948. Variations in the properties of potato virus X and their effects on its interaction with ribo-nuclease and proteolytic enzymes. J. Gen. Microbiol. 2: 173–185.

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Authors
  1. James Munro
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Additional information

Contribution No. 229, Research Station, Canada Department of Agriculture, Fredericton, New Brunswick, Canada.

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Munro, J. Use of molecular gel column to prepare potato virus X inocula for antisera. American Potato Journal 44, 249–254 (1967). https://doi.org/10.1007/BF02862521

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  • DOI: https://doi.org/10.1007/BF02862521

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