Economic Botany

, Volume 47, Issue 1, pp 79–88 | Cite as

Cultivation ofPhyllanthus amarus and evaluation of variables potentially affecting and the inhibition of viral DNA polymerase

  • David W. Unander
  • Herbert H. Bryan
  • Connie J. Lance
  • Robert T. Mcmillan


Phyllanthus amarus (Euphorbiaceae) possesses activity against hepatitis B virus and related hepadnaviruses. One such activity, the inhibition of endogenous hepadnavirus DNA polymerase, differed little between cultivatedP. amarus and plants collected from the wild. Inhibitory activity equivalent to that in wild plants was obtained from both shoots and roots sown at different times of the year in a subtropical region (Dade County, Florida, U.S.A.). Plant size and two levels of fertilization did not significantly affect activity. Plots were planted using a cellulose gel to evenly disperse the small seeds. Gel amendments used at sowing had no significant effect on the antiviral activity of harvested plants. Drip irrigation permitted successful cultivation during the dry season. Plastic mulch was used to control weeds.Phyllanthus amarus grows slowly, and reaches a maximum size and vigor at about 5–7 months after sowing. Under south Florida conditions, the greatest biomass ofP. amarus was produced when seeds were sown in mid-winter for a summer harvest. With weed control by the mulch, water and fertilization via the drip irrigation, six and a half month old plants (from sowing) reached an average dry weight of approximately 40 glplant when harvested in July or August.

Key Words

Phyllanthus amarus hepatitis B virus woodchuck hepatitis virus antivirals gel seeding humic acid Glomus spp 

El cultivo dePhyllanthus amarus y la evaluatión de variables que potencialmente afectan el rendimiento y la inhibitión de la DNA polimerasa viral


Phyllanthus amarus (Euphorbiaceae) posee actividades contra el virus hepatitis B y otros hepadnavirus relacionados. Una actividad específica, la inhibición de la polimerasa DNA endógena de los hepadnavirus, no varió entre las plantas cultivadas y colecciones de plantas silvestres. Un nivel de actividad inhibitoria equivalente a plantas silvestres se obtuvo de vástagos y raices de plantas sembradas en diferentes epocas del ano en una región subtropical (Dade County, Florida, EE UU). El tamaño de la planta y dos niveles de abonamiento no tuvo efecto significativo sobre la actividad. Las parcelas fueron sembrados usando un gel de celulosa para dispersar homogéneamente las pequeñas semillas. Diferentes enmiendas de gel al sembrar no tuvieron efecto sobre la actividad antiviral de las plantas cosechadas. El uso de riego por goteo permitio el cultivo exitoso durante la temporada seca. Se usó plástico para controlar las malezas. El crecimiento deP. amarus es lento, y llega asu máximo tamaño y vigor entre los 5 y 7 meses después de la siembra. Bajo las condiciones del sur de la Florida, se produjo la máxima bíomasa cuando se sembró en medio del invierno para cosechar en el verano. Por medio del uso de plástico para controlar malezas, riego por goteo y la fertilizatión a través del riego, el promedio de peso seco por planta de seis meses y medio de edad fue de 40 g al cosecharlas en julio o agosto.


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Copyright information

© The New York Botanical Garden 1993

Authors and Affiliations

  • David W. Unander
    • 1
  • Herbert H. Bryan
    • 3
  • Connie J. Lance
    • 3
  • Robert T. Mcmillan
    • 2
    • 3
  1. 1.Division of Population ScienceFox Chase Cancer CenterPhiladelphiaUSA
  2. 2.Department of BiologyEastern CollegeSt. Davids
  3. 3.Institute for Food and Agricultural SciencesUniversity of Florida, Tropical Research and Education CenterHomesteadUSA

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