Archives of Pharmacal Research

, 10:158 | Cite as

Studies on protoplast formation and regeneration ofGanoderma lucidum

  • Seung Hee Choi
  • Byong Kak Kim
  • Ha Won Kim
  • Jin Hwan Kwak
  • Eung Chil Choi
  • Young Choong Kim
  • Young-Bok Yoo
  • Yong Hwan Park
Original Articles


To obtain a new strain ofGanoderma lucidum by protoplast fusion technique, its protoplast formation and regeneration were studied. Several factors affecting the protoplast formation and regeneration were investigated to find their optimum conditions. The mycelium was grown for four days on the cellophane membrane placed onG. lucidum complete medium (GCM). When various commercial lytic enzymes were examined for protoplast isolation, the combination of Novozym 234 and β-glucuronidase was found to be effective. An osmotic stabilizer, 0.6 M sucrose in 20 mM phosphate buffer pH 5.8, gave the highest yield of protoplasts. Three-hour incubation in shaking incubator was most suitable for releasing protoplasts. To increase the protoplast yield, pretreatment with 2-mercaptoethanol was carried out. The regeneration frequency in GCM containing 0.6 M MgSO4·7H2O was shown to be 0.66%.


Ganoderma lucidum Polyporaceae Protoplast Lytic enzyme Osmotic stabilizer Regeneration frequency 

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Copyright information

© The Pharmaceutical Society of Korea 1987

Authors and Affiliations

  • Seung Hee Choi
    • 1
  • Byong Kak Kim
    • 1
  • Ha Won Kim
    • 1
  • Jin Hwan Kwak
    • 1
  • Eung Chil Choi
    • 1
  • Young Choong Kim
    • 1
  • Young-Bok Yoo
    • 2
  • Yong Hwan Park
    • 2
  1. 1.College of PharmacySeoul National UniversitySeoul
  2. 2.Institute of Agricultural SciencesSuwonKorea

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