Fertilization of IVF/ICSI using sibling oocytes from couples with subfertile male or unexplained infertility
- 52 Downloads
The significance of the performance of conventionalin vitro fertilization and intracytoplasmie sperm injection (IVF/ICSI) using sibling oocytes from couples with subfertile male or unexplained infertility was evaluated. A total of 410 sibling oocyte cumulus-corona complexes (OCCC) from 21 couples with subfertile male (group A) and 11 unexplained infertile couples (group B) were randomly divided, in order of retrieval, into two groups inseminated either by conventional IVF or by ICSI. The treatment outcomes and the influence of infertility factors on fertilization in each group were compared. The results showed that although the two pronuclear (2PN) fertilization rate per injected sibling oocytes was significantly higher after ICSI (group A: 68.2%±28.8%; group B: 66.2% ± 24.9%) than after conventional IVF (group A: 41.8%±32.7%; group B: 40.1% ±22.1%), the other variables studied included: the fertilization rates of per allocated sibling oocytes IVF/ICSI, the fertilization rates of sibling oocytes IVF/ICSI after excluding failed IVF fertilization cycles, as well as the eleavage rates of normal fertilization were not statistically significant (P <0.05). Similarly, though the total fertilization failure rate in the IVF group (group A: 42.9%; group B: 36.4%) was significantly higher than in the ICSI group (group A: 4.8%; group B: 0), we did not cancel cycles due to the normal fertilization of sibling oocytes. Embryo transfer was possible in all 32 couples. There were 10 clinical pregnancies in the two groups. We also discovered a possible association between some semen parameters and sperm functions of group A, and women age and duration of infertility of group B and fertilization. It is suggested that adoption of the split IVF/ICSI technology in the above cases may help eliminate fertilization failures. This is also a useful method to investigate the effect of single factor on the employment of assisted reproductive technology.
Key wordssibling oocytes in vitro fertilization intracytoplasmic sperm injection subfertile male infertility unexplained infertility
Unable to display preview. Download preview PDF.
- 4.WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. New York: Cambridge University Press, 1999. 51–2Google Scholar
- 5.Huang Y F, Xu R J. The diagnosis of male factor infertility. Shanghai: Second Military Medical University Publishing House, 1999, 146–8Google Scholar