Abstract
Effects of basic fibroblast growth factor (bFGF) and insulin-like growth factor II (IGF-II) on cartilage cells from proboscis of skate,Raja porasa Günther, were investigated in this study. The cartilage cells were cultured in 20% FBS-supplemented MEM medium at 24°C. Twelve hours after culture initiation, the cartilage cells were treated with bFGF and IGF-II at different concentration combinations. It was found that 20 ng/ml of bFGF or 80 ng/ml of IGF-II was enough to have obvious stimulating effect on the growth and division of skate cartilage cells. Test of bFGF and IGF-II together, revealed that 20 ng/ml of bFGF and 80 ng/ml of IGF-II together had the best stimulating effect on the growth and division of skate cartilage cells. The cartilage cells cultured could form a monolayer at day 7.
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This work was partially supported and funded by the China Education Committee as the “Imbursement Project for Studied Abroad Returnees”.
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Tingjun, F., Lingyun, J. & Xiaofeng, W. Effects of basic fibroblast growth factor and insulin-like growth factor on cultured cartilage cells from skateRaja porasa . Chin. J. Ocean. Limnol. 21, 305–311 (2003). https://doi.org/10.1007/BF02860424
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DOI: https://doi.org/10.1007/BF02860424