Effects of aloe extracts on human normal and tumor cells in vitro
- 221 Downloads
Fractions of leaf extracts from 2 local types, labeledAloe vera (subsequently identified asAloe barbadensis Mill, andA. saponaria Haw.), were prepared by differential centrifugation and tested by in vitro assays for the presence of lectinlike activities and for effects on the attachment and growth of human normal and tumor cells. Fractions of extracts of fresh leaves and commercially “stabilized”Aloe vera gel had high levels of lectin-like substances measured by immunodiffusion and hemagglutination assays. Substances in fluid fractions from both fresh leaf sources were found to markedly promote attachment and growth of human normal, but not tumor, cells and to enhance healing of wounded cell monolayers. In contrast, fractions of “stabilized”Aloe vera gel were equally cytotoxic for human normal and tumor cells in vitro. Results from cell assays suggested that the observed growth promotion and wound healing effects of aloe substances in vitro may be analogous to what has been observed in vivo during healing of wounds and burns.
KeywordsEconomic Botany Aloe Substance ME180 Cell Aloe Barbadensis Aloe Vera Extract
Unable to display preview. Download preview PDF.
- Bouchey, G. D., and G. Gjerstad. 1969. Chemical studies ofAloe vera (barbadensis) juice, II. Inorganic ingredients. Quart. J. Crude Drug Res. 9: 1445–1453.Google Scholar
- Crewe, J. R. 1939. Aloes in the treatment of burns and scalds. Minnesota Medicine 22: 538–539.Google Scholar
- Gjerstad, G. 1971. Chemical studies ofAloe vera juice. I. Amino acid analysis. Advancing Frontiers PL. Sci. 28: 311–315.Google Scholar
- Rowe, T. D., and L. M. Parks. 1941. A phytochemical study ofAloe vera leaf. J. Amer. Pharm. Assoc. 30: 262–266.Google Scholar
- —, B. K. Lowell, and L. M. Parks. 1941. Further observations on the use ofAloe vera leaf in the treatment of third degree X-ray reactions. J. Amer. Pharm. Assoc. 30: 266–269.Google Scholar
- Ship, A. G. 1977. Is topicalAloe vera plant mucus helpful in burn treatment? J. Amer. Med. Assoc. 238: 1770–1772.Google Scholar
- Suzuki, I., H. Saito, S. Inuoe, S. Migita, and T. Takahashi. 1979. Purification and characterization of two lectins fromAloe aborescens. Mill. J. Biochem. 85: 163–171.Google Scholar
- Winters, W. D. 1979. Human adenovirus antibody in sera of normal and tumour-bearing dogs. Veterin. Record. 105: 216–220.Google Scholar