Abstract
Tuber proteins of oca (Oxalis tuberosa) were separated for clone (cultivar) differentiation by gel electrophoresis in polyacrylamide. The patterns of native proteins and especially of esterases in a porosity gradient with and without urea were suitable for differentiation, whereas SDS-electrophoresis of protomers or focusing of native proteins yielded more or less the same patterns for 23 different accessions. The main proteins are quite acidic (isoelectric points pH 4.7–4.9) and have a relative molecular weight of 17–18 KD (Kilo-Dalton). Amino acid analysis of the protein precipitate by propan-2-ol from tuber sap revealed proteins rich in aspartic and glutamic acid (one third of all amino acids) with a considerable share of essential ones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature Cited
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein. Analytical Biochem. 72:248–254.
de Wreede, I., and H. Stegemann. 1981. Determination of proteins by a modified biuret-method. Fresenius Z. Analytische Chem. 308:431–433.
Gibbs, P. E. 1976. Studies on the breeding system of Oxalis tuberosa Mol. Flora 165:129–138.
—, D. Marshall, and D. Brunton. 1978. Studies on the cytology of Oxalis tuberosa and Tro paeolum tuberosum. Notes Roy. Bot. Gard. Edinburgh 37:15–220.
Hill, A. W. 1939. XVIII-The oca and its varieties. Bull. Misc. Inform. Roy. Bot. Gard. Kew No. 4:169–173.
Hodge, W. H. 1946. Three neglected Andean tubers. J. New York Bot. Gard. 47:213–224.
King, S. 1986. Ancient buried treasure of the Andes. Garden 10(6):6–11.
León, J. 1964. Plantas alimenticias Andinas. Bol. Técn. Inst. Interamer. Ci. Agric., Lima, Peru.
OSE. 1979. Anuario estadístico agrícola. Ministerio de Agriculture. Oficina Sectorial de Estadística. Lima, Peru.
Stegemann, H. 1963. Proteine (Conchagene) und Chitin im Stützgewebe von Tintenfischen. Hoppe- Seyler’s Z. Physiol. Chem. 331:269–279.
—. 1984. Retrospect on 25 years of cultivar identification by protein patterns. Pages 20–31 in S. R. Draper and R. J. Cooke, eds., Biochemical tests for cultivar identification. Int. Seed Testing Assoc., Zürich.
—, and G. Bernhard. 1961. Die Trennung und Analyse von Aminosäuren im Ultramikromaßstab. Mikrochim. Acta 1961:555–563.
-, W. Burgermeister, C. Ochoa, and S. Huaman. 1985. Lecture: the collection of 13000 primitive potato cultivars in Lima and its cutback to about 2000 truly distinct samples by PAGE, 2nd Internat. Symposion 9 May 1985 in Braunschweig “Biochemical Approaches to Identification of Cultivars and Evaluation of Their Properties,” p. 5.
—, H. Francksen, and V. Macko. 1973. Potato proteins: genetic and physiological changes, evaluated by one- and two-dimensional PAA-gel-techniques. Z. Naturf. 28c:722–732.
—, and P. Schmiediche. 1981. Proteinmuster von Sauerklee (Oxalis tuberosa). Jahresber. Biol. Bundesanst. H:111.
—, and D. Schnick. 1982. Index 1982 of European potato varieties. Mitt. Biol. Bundesanst. 211:1–220.
Tapia, M. E. 1984. Avances en las investigaciones sobre tuberculos alimenticios de los Andes. Agriculture Andina, Proyecto PISCA IICA/CIID.
Author information
Authors and Affiliations
Additional information
The results given in this paper were presented as a poster during the 2nd International Symposion, 5–9 May 1985, “Biochemical Approaches to Identification of Cultivars and Evaluation of Their Properties” in Braunschweig, Abstract issue, p. 16.
Rights and permissions
About this article
Cite this article
Stegemann, H., Majino, S. & Schmiediche, P. Biochemical differentiation of clones of OcaOxalis tuberosa, Oxalidaceae) by Their tuber proteins and the properties of these proteins. Econ Bot 42, 37–44 (1988). https://doi.org/10.1007/BF02859028
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02859028