Archives of Pharmacal Research

, Volume 11, Issue 2, pp 139–144 | Cite as

Microbial aspartase and its activity on deamination of L-aspartyl-L-phenylalanine methyl ester

  • Wonyoon Chung
  • Yang Mo Goo
Original Articles


Examination of many microorganisms and soil isolates for the activity of aspartase proved thatR. rubra, G. suboxydans, A. versicolor, P. purpurogenum, E. coli, Ps. aeruginosa, A. gigantus, A. unguis, A. parasiticus and a soil isolate(S-90) had high activity of aspartase. Comparision of the activity of the aspartase by cell free extracts of these micro-organisms with the activity of the enzyme catalyzing the deamination of aspartame by the same cell free extracts showed similar kinetic characteristics. The aspartase existing in the cell free extracts seemed to catalyze the deamination of aspartame, too.


screening of aspartase L-aspartic acid aspartame activity of L-aspartylphenyl-alanine methyl ester ammonia lyase 


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Copyright information

© The Pharmaceutical Society of Korea 1988

Authors and Affiliations

  • Wonyoon Chung
    • 1
  • Yang Mo Goo
    • 1
  1. 1.College of PharmacySeoul National UniversitySeoulKorea

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