Advertisement

Protective effects of ciliary neurotrophic factor on denervated skeletal muscle

  • Huang Shilong
  • Wang Fabin
  • Hong Guangxiang
  • Wan Shengxiang
  • Kang Hao
Article

Summary

To study the effects of ciliary neurotrophic factor (CNTF) on denervated skeletal muscle atrophy and to find a new approach to ameliorate atrophy of denervated muscle, a model was established by cutting the right sciatic nerve in 36 Wistar mice, with the left side serving as control. Then they were divided into two groups randomly. CNTF (1 U/ml) 0.1 ml was injected into the right tibial muscle every day in experimental group, and saline was used into another group for comparison. The muscle wet weight, muscle total protein, Ca2+, physiological response and morphology were analyzed on the 7th, 14th and 28th day after operation. Our results showed that compared to control group, there was a significant increase in muscle wet weight, total protein, Ca2+, muscle fiber cross-section area in CNTF group (P<0.05). CNTF could ameliorate the decrease of tetanic tension (PO), post-tetanic twitch potentiation (PTP), and the prolonged muscle relaxation time (RT) caused by denervation (P<0.05). The motor end-plate areas 7 days and 14 days after denervation was similar (P>0.05), but significantly larger 28 days after the denervation (P<0.05). Our results suggest that CNTF exerts myotrophic effects by attenuating the morphological and functional changes associated with denervation of rat muscles and has protective effects on denervated muscle and motor end plate.

Key words

peripheral nerve skeletal muscle atrophy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gurney M E, Yamamoto H, Kwon Y. Induction of motor neuron sprouting in vivo by ciliary neurotrophic factor and basic fibroblast growth factor. J Neurosci, 1992, 12: 324Google Scholar
  2. 2.
    Davis S, Aldrich T H, Valenzuela D M. The receptor for ciliary neurotrophic factor. Science, 1991, 253: 59PubMedCrossRefGoogle Scholar
  3. 3.
    Weis J, Lie D C, Ragoss U. Increased expression of CNTF receptor alpha in denervated human skeletal muscle. J Neuropathol Neurol, 1998, 57: 850CrossRefGoogle Scholar
  4. 4.
    Card D J. Denervation: sequence of neuromuscular degenerative changes in rats and the effects of stimulation. Exp Neurol, 1997, 54: 251CrossRefGoogle Scholar
  5. 5.
    Brimijoin S. Trophic roles of axoplasmic transport in nerve-muscle cell trophic communication. HL Fernandez and JA Donoso, eds. Boca Raton, Florida: CRC Press, 1991. p23–29Google Scholar
  6. 6.
    Wheeler E F, Bothwell M. Spatiotemporal patterns of expression of NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogemesis. J Neurosci, 1992, 12: 930PubMedGoogle Scholar
  7. 7.
    Funakoshi H, Belluardo N, Arenas Eet al. Muscle-derived neurotrophin-4 as an activity-dependent trophic signal for adult motor neurons. Science, 1995, 268: 1495PubMedCrossRefGoogle Scholar
  8. 8.
    Finkelstein D I, Bartlett P F, Horne M K. Leukemia inhibitory factor is a myotrophic and neurotrophic agent that enhance the reinnervation of muscle in the rat. J Neurosci Res, 1996, 46 (1): 122PubMedCrossRefGoogle Scholar
  9. 9.
    Davis S, Aldrich T H, Stahl Net al. LIFR beta and gp130 as heterodimerizing singal transducers of the tripartite CNTF receptor. Science, 1993, 260 (5115): 1805PubMedCrossRefGoogle Scholar
  10. 10.
    Davis H L, Kiernan J A. Effect of nerve extract on atrophy of denervated or immobilized muscles. Exp Neurol, 1981, 72: 582PubMedCrossRefGoogle Scholar
  11. 11.
    Davis H L. Myotrophic effects on denervation atrophy of hindlimb muscles of mice with systemic administration of nerve extract. Brain Res, 1985, 343: 176PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2002

Authors and Affiliations

  • Huang Shilong
    • 1
  • Wang Fabin
    • 1
  • Hong Guangxiang
    • 1
  • Wan Shengxiang
    • 1
  • Kang Hao
    • 1
  1. 1.Department of Hand Surgery, Xiehe Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

Personalised recommendations