Effects of antitumor polysaccharides fromAlbizza julibrissin on immune function

  • Chang Kiu Moon
  • Byeong Gon Lee
  • Soo Hwan Lee
  • Tak Lim Kang
Original Articles


Polysaccharide fractions were prepared fromAlbizzia julibrissin by different extraction schedules. The fractions obtained were designated as PS-I and PS-II, respectively. Further purification of PS-I by Sephadex G-200 column chromatography gave two subfractions, Alju A and Alju B. Each fraction showed marked antitumor activity against sarcoma 180 solid form but not ascite form. PS-I and PS-II increased delayed hypersensitivity in normal and tumor bearing mice. PS-I treatment led to moderate restoration of the suppressed antibody production in tumor bearing mice.


Polysaccharide Albizzia julibrissin Antitumor activity Hemolytic plaque assay Delayed hypersensitivity test 

Literature Cited

  1. 1).
    Maeda, Y.Y., and Chihara, G.: The effects of neonatal thymectomy on the antitumor activity of lentinan, carboxymethylpachymaran and zymosan and their effects on various immune responses.Int. J. Cancer;11, 153–161 (1973).PubMedCrossRefGoogle Scholar
  2. 2).
    Chihara, G., Hamuro, J., Maeda, Y.Y., Arai, Y., and Fukuoka, F.: Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, fromLentinus edodes (Berk.) sing. (an edible mushroom).Cancer Res.,30, 2776–2781 (1970).PubMedGoogle Scholar
  3. 3).
    Saito M. et al.: Activated macrophages are responsible for the tumor inhibitory effect in mice receiving intravenous injection of OK-432.Int. J. Cancer,15, 33(2), 271–6, Feb. (1984).CrossRefGoogle Scholar
  4. 4).
    Yoshikumi, C., Nomoto, K., Matsunaga, K., Fujii, T., and Takeya, K.: Mouse strain difference in the expression of antitumor activity of PS-K.Gann,66, 649–654; Dec. (1975).PubMedGoogle Scholar
  5. 5).
    Kohno, M., Abe, S., Nakayama, H., Yamazaki, M., and Misuno, D.: Comparative studies on immunological properties of antitumor polysaccharde TC-13.Gann,73, 618–626; Aug., (1982).PubMedGoogle Scholar
  6. 6).
    Moon, C.K., Sim, K.S., Lee, S.H., Park, K.S., Yoon, Y.P., Ha, B.J., and Lee, C.C.: Antitumor activity of some phytobased polysaccharides and their effects on the immune function.Arch. Pharm. Res. 6 (2), 123–131 (1983).Google Scholar
  7. 7).
    Moon, C.K., Park, K.S., Lee, S.H., and Yoon, Y.P.: Antitumor activities of several phytopolysaccharides.Arch. Pharm. Res. 8 (1), 42–44 (1985).Google Scholar
  8. 8).
    Moon, C.K., Park, K.S., Lee, S.H., Ha, B.J., and Lee, B.G.: Effects of antitumor polysaccharides fromForsythia corea on the immune function (I).Arch. Pharm. Res. 8 (1), 31–38 (1985).CrossRefGoogle Scholar
  9. 9).
    Caldes, G., Prescott, B.: Thomas II, C.A., and Baker P.J.: Characterization of a polysaccharide fromCarthamus tinctorius that cross reacts with type III pneumococcal polysaccharide.J. Gen. Appl. Microbiol.,27, 157–172 (1981).CrossRefGoogle Scholar
  10. 10).
    Terrance, G.C.:The tools of biochemistry, John Wiley & Sons N.Y. p. 169 (1977).Google Scholar
  11. 11).
    Cunningham, A.J., and Szenberg, A.: Further improvements in the plaque technique for detecting single antibody forming cells.Immunology,14, 599, (1968).PubMedGoogle Scholar
  12. 12).
    Titus, R.G., and Chiller, J.M.: A simple and effective method to assess murine delayed type hypersensitivity to proteins.J. Immunol. Method. 45, 65–78 (1981).CrossRefGoogle Scholar
  13. 13).
    Ohno, R., Yokomaku, S., Wakayama, K., Sugiura, S., Ima, K., and Yamada, K.: Effect of protein-bound polysaccharide preparation, PS-K, on the immune response of mice to sheep red blood cells.Gann,67, 97–99; Feb (1976).PubMedGoogle Scholar
  14. 14).
    Masuko, Y., Nakajima, H., Tsubouchi, J., Yamazaki, M., Mizuno, D., and Abe, S.: Changes of antitumor immunity of hosts with murine mammary tumors regressed by Lentinan: potenti ation of antitumor delayed hypersentisitivy reaction.Gann 73, 790–797; oct. (1982).PubMedGoogle Scholar
  15. 15).
    Shinoda, M., Yamazaki, M., and Mizuno, D.: Protective effect of syngeneic antitumor serum on mammary tumor in a syngeneic host.Gann 68, 561–565 (1977).PubMedGoogle Scholar
  16. 16).
    Abe, S., Yamazaki, M., and Mizuno, D.: Correlation of differences in antigenicity of four 3-Methylcholanthrene induced tumors in syngeneic mice with the susceptibility of tumors to an immunopotentiator, PS-K.Gann,69, 223–228 (1978).PubMedGoogle Scholar
  17. 17).
    Kurius, M., Yamazaki, M., and Mizuno, D.: Induction of Macrophage mediated tumorlysis by the Lectin wheat germ agglutinin.Cancer Res.,40, 3798 (1980).Google Scholar
  18. 18).
    Taffet, S.M., and Russell, S.W.: Macrophage mediated tumor cell killing; Regulation of expre ssion of cytolytic activity by prostaglandin E.J. Immunol. 126, 424 (1981).PubMedGoogle Scholar
  19. 19).
    Sudol, A.A., and Nathan, C.F.: Suppression of macrophage oxidative metabolism by products of malignant and nonmalignant cells.J. Exp. Med. 156, 945–961 Oct. (1982).CrossRefGoogle Scholar
  20. 20).
    Saito, M., Yamazaki, M., and Misuno, D.: Immune response of mice in immunotherapy of tumors with syngeneic antitumor serum plus lipopolysaccharide.Gann,69, 331–337; June. (1978).PubMedGoogle Scholar
  21. 21).
    Piccolella, E., Lombardi, G., and Morelli, R.: Human lymphocyte activating properties of a purified polysaccharade fromCandida albicans; B and T cell cooperation in the mitogenic response.J. Immunol.,125, 2082 (1980).PubMedGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 1985

Authors and Affiliations

  • Chang Kiu Moon
    • 1
  • Byeong Gon Lee
    • 1
  • Soo Hwan Lee
    • 1
  • Tak Lim Kang
    • 2
  1. 1.College of PharmacySeoul National UniversitySeoulKorea
  2. 2.Department of Oriental MedicineDae-Jeon CollegeDae-JeonKorea

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