Comparison of different staining methods for analysing esterase (EST) isozyme of fungi and plants
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EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi, and the staining is an important process in electrophoresis analysis of studying Est. The effects were compared among different stain recipes for Est of 3 kinds of fungi-Lentinusedodes,Pleurotus sapidus, Phellinus igriarius and 2 kinds ofplants-Populus sp andBrassica chinensis. Of the four kinds of Est staining recipes tested, the recipe α-acetic acid-naphther showed the best effect. and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacid-nathpher.
Key wordsFungi Esterase isozyme Staining methods
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