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Journal of Northeast Forestry University

, Volume 7, Issue 4, pp 24–27 | Cite as

Comparison of different staining methods for analysing esterase (EST) isozyme of fungi and plants

  • Chi Yujie
  • Zhang Ming
  • Cheng Dongsheng
  • Xu Cuiqing
Article
  • 61 Downloads

Abstract

EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi, and the staining is an important process in electrophoresis analysis of studying Est. The effects were compared among different stain recipes for Est of 3 kinds of fungi-Lentinusedodes,Pleurotus sapidus, Phellinus igriarius and 2 kinds ofplants-Populus sp andBrassica chinensis. Of the four kinds of Est staining recipes tested, the recipe α-acetic acid-naphther showed the best effect. and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacid-nathpher.

Key words

Fungi Esterase isozyme Staining methods 

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References

  1. 1.
    Cheng, D. S. Differentiation among Cronartium species from Northeast China by Isozyme Analysis, Proc. 4th IUFRO Rusts of Pines Working Party Conf. 71–75, 1995.Google Scholar
  2. 2.
  3. 3.
    1986, (2) 7–8Google Scholar

Copyright information

© Northeast Forestry University 1996

Authors and Affiliations

  • Chi Yujie
    • 1
  • Zhang Ming
    • 1
  • Cheng Dongsheng
    • 1
  • Xu Cuiqing
    • 1
  1. 1.Northeast Forestry UniversityHarbinChina

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