American Journal of Potato Research

, Volume 76, Issue 2, pp 97–102 | Cite as

A genetic map ofSolanum phureja clone 1.22 constructed using RFLP and RAPD markers

  • Kazuyoshi Hosaka


A genetic map of a widely used haploid inducer,Solanum phureja clone 1.22, was constructed. One hundred and twenty-two RFLP and twenty RAPD marker bands were specific toS. phureja clone 1.22, and were mapped on 100 loci (five to eleven loci per chromosome) in the span of 606 cM. These would be useful to monitor the chromosomes of the haploid inducer that may be eliminated from triploid embryos of 4x × 2x during the process of haploid induction from potato.

Additional Key Words

RFLP RAPD haploid inducer linkage map chromosome elimination 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Bonierbale, M.W., R.L. Plaisted, and S.D. Tanksley. 1988. RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103.PubMedGoogle Scholar
  2. Clulow, S.A., M.J. Wilkinson, and L.R. Burch. 1993.Solanum phureja genes are expressed in the leaves and tubers of aneusomatic potato dihaploids. Euphytica 69:1–6.CrossRefGoogle Scholar
  3. Clulow, S.A., M.J. Wilkinson, R. Waugh, E. Baird, M.J. DeMaine, and W. Powell. 1991. Cytological and molecular observations onSolanum phureja-induced dihaploid potatoes. Theor Appl Genet 82:545–551.CrossRefGoogle Scholar
  4. Gebhardt, C., E. Ritter, T. Debener, U. Schachtschabel, B. Walkemeier, H. Uhrig, and F. Salamini. 1989. RFLP analysis and linkage mapping inSolanum tuberosum. Theor Appl Genet 78:65–75.CrossRefGoogle Scholar
  5. Gebhardt, C., E. Ritter, A. Barone, T. Debener, B. Walkemeier, U. Schachtschabel, H. Kaufmann, R.D. Thompson, M.W. Bonierbale, M.W. Ganal, S.D. Tanksley, and F. Salamini. 1991. RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57.CrossRefGoogle Scholar
  6. Hermsen, J.G.Th. and J. Verdenius. 1973. Selection fromSolanum tuberosum Group Phureja of genotypes combining high-frequency haploid induction with homozygosity for embryo-spot. Euphytica 22:244–259.CrossRefGoogle Scholar
  7. Hermundstad, S.A. and S.J. Peloquin. 1985. Germplasm enhancement with potato haploids. J Hered 76:463–467.Google Scholar
  8. Hosaka, K. and D.M. Spooner. 1992. RFLP analysis of the wild potato species,Solanum acaule Bitter (Solanum sect.Petota). Theor Appl Genet 84:851–858.CrossRefGoogle Scholar
  9. Hosaka, K. and R.E. Hanneman, Jr. 1994. Random amplified polymorphic DNA markers detected in a segregating hybrid population ofSolanum chacoense xS. phureja. Jpn J Genet 69:53–66.CrossRefGoogle Scholar
  10. Hosaka, K. and R.E. Hanneman, Jr. 1998. Genetics of self-compatibility in a self-incompatible wild diploid potato speciesSolanum chacoense. 2. Localization of an S locus inhibitor (Sli) gene on the potato genome using DNA markers. Euphytica 103:265–271.CrossRefGoogle Scholar
  11. Hougas, R.W., S.J. Peloquin, and A.C. Gabert. 1964. Effect of seed-parent and pollinator on frequency of haploids inSolanum tuberosum. Crop Sci 4:593–595.CrossRefGoogle Scholar
  12. Jacobs, J.M.E., H.J. Van Eck, P. Arens, B. Verkerk-Bakker, B. te Lintel Hekkert, H.J.M. Bastiaanssen, A. El-Kharbotly, A. Pereira, E. Jacobsen, and W.J. Stiekema. 1995. A genetic map of potato (Solanum tuberosum) integrating molecular markers, including transposons, and classical markers. Theor Appl Genet 91:289–300.CrossRefGoogle Scholar
  13. Kosambi, D.D. 1944. The estimation of map distance from recombination values. Ann Eugen 12:172–175.Google Scholar
  14. Kotch, G.P. and S.J. Peloquin. 1987. A new source of haploid germplasm for genetic and breeding research. Am Potato J 64:137–141.CrossRefGoogle Scholar
  15. Kotch, G.P., R. Ortiz, and S.J. Peloquin. 1992. Genetic analysis by use of potato haploid populations. Genome 35:103–108.Google Scholar
  16. Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln, and L. Newburg. 1987. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181.PubMedCrossRefGoogle Scholar
  17. Misoo, S., K. Matsuda, T. Yamada, K. Komura, M. Chaya, and O. Kamijima. 1997. Chromosome configuration of haploid level progeny derived from crossing potato cv. Chijiwa withSolanum phureja. Breed Sci 47(Suppl. 2):370.Google Scholar
  18. Peloquin, S.J., Gabert, A.C., and R. Ortiz. 1996. Nature of ‘pollinator’ effect in potato (Solanum tuberosum L.) haploid production. Ann Bot 77:539–542.CrossRefGoogle Scholar
  19. Peloquin, S.J., G.L. Yerk, J.E. Werner, and E. Darmo. 1989. Potato breeding with haploids and2n gametes. Genome 31:1000–1004.Google Scholar
  20. Ross, H. 1986. Potato breeding—problems and perspectives. Verlag Paul Parey, Berlin and Hamburg, pp 132.Google Scholar
  21. Tanksley, S.D., M.W. Ganal, J.P. Prince, M.C. de Vicente, M.W. Bonierbale, P. Broun, T.M. Fulton, J.J. Giovannoni, S. Grandillo, G.B. Martin, R. Messeguer, J.C. Miller, L. Miller, A.H. Paterson, O. Pineda, M.S. Röder, R.A. Wing, W. Wu, and N.D. Young. 1992. High density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160.PubMedGoogle Scholar
  22. Wangenheim, K.-H.V., S.J. Peloquin, and R.W. Hougas. 1960. Embryological investigations on the formation of haploids in the potato (Solanum tuberosum). Z Vererbgslhre 91:391–399.CrossRefGoogle Scholar
  23. Wilkinson, M.J., S.T. Bennett, S.A. Clulow, J. Allainguillaume, K. Harding, and M.D. Bennett. 1995. Evidence for somatic translocation during potato dihaploid induction. Heredity 74:146–151.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 1999

Authors and Affiliations

  1. 1.Experimental FarmKobe UniversityHyogoJapan

Personalised recommendations