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American Potato Journal

, Volume 64, Issue 3, pp 97–108 | Cite as

Comparison of three potato leafroll virus antisera and a single Beet western yellows virus antiserum for luteovirus detection in potato

  • Dale J. Gallenberg
  • Thomas A. Zitter
  • Edward D. Jones
Article

Abstract

Three potato leafroll virus (PLRV) antisera, representing European, British Columbian, and Californian isolates, performed similarly in detection of PLRV in ELISA tests of samples collected in three successive years at the Florida certification test plots and in tests of other samples collected in New York State. Although a range of absorbance values occurred, this was probably due to random variation in virus titers of samples rather than the occurrence of different virus strains or differential serological reactions by the antisera. Beet western yellows virus (BWYV) was detected in potato leafroll samples from nine states and provinces in North America. The BWYV-positive samples represented 40% in 1983 and 62.5% in 1984 of the total number of samples tested. These results confirm previous reports on the widespread occurrence of BWYV in potato with symptoms of leafroll.

Key Words

ELISA serological detection potato viruses 

Resumen

Tres antisueros del virus del enrollamiento de la hoja de la papa (PLRV), representando aislamientos europeos, de la Columbia Británica, y de California, se comportaron similarmente en la detección del PLRV en pruebas ELISA de muestras recolectadas durante tres años sucesivos en las parcelas para pruebas de certificación en Florida y en pruebas de otras muestras recolectadas en el estado de Nueva York. Si bien se observó un amplio rango de valores de absorbencia, éste probablemente se debió a la variación obtenida al azar en las dosificaciones de los virus en las muestras, más que por la presencia de variantes o de reacciones serológicas diferentes de los antisueros. El virus del amarillamiento occidental de la betarraga (BWYV) fue detectado en muestras del enrollamiento de la papa correspondientes a nueve estados y provincias de los Estados Unidos. Las muestras positivas de BWYV representaron 40% en 1983, y 62,5% en 1984, del número total de muestras probadas. Estos resultados confirman los informes existentes sobre la amplia distribución del BWYV en papas con síntomas de PLRV.

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Copyright information

© Springer 1987

Authors and Affiliations

  • Dale J. Gallenberg
    • 1
  • Thomas A. Zitter
    • 1
  • Edward D. Jones
    • 1
  1. 1.Dept. of Plant PathologyCornell UniversityIthaca

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