Loss of potato spindle tuber viroid from tuber tissues after repeated freezing
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Potato spindle tuber viroid (PSTV) infected foliage and tuber tissues when subjected to repeated freezing and thawing under controlled conditions (−18 to − 20 C and 5 C), lost PSTV more rapidly in tuber tissues than in foliage. Tubers of 17 potato cultivars showed similar tendencies, but with some differences. In 7 of the 17 cultivars, PSTV was significantly reduced after one freeze-thaw treatment, and in 6 of the 17 cultivars, infectious PSTV was still recoverable after exposure to 4 cycles of freeze-thaw. Exposure of PSTV-infected tubers to outdoor temperature fluctuations (13.1 C to −15.1 C) resulted in complete loss of PSTV from the tubers.
Additional Key WordsReturn-polyacrylamide gel electrophoresis nucleic acid hybridization strains detection elimination
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