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American Potato Journal

, Volume 63, Issue 7, pp 357–362 | Cite as

Comparison of latex agglutination, enzyme-linked immunosorbent assay, and indicator plants for detection of potato viruses S and X in potatoes

  • Gary D. Franc
  • Ernest E. Banttari
Article
  • 22 Downloads

Abstract

Potato virus S (PVS) was consistently detected by the enzyme-linked immunosorbent assay (ELISA) and the latex agglutination test (LAT) at dilutions (PVS sap: healthy sap) of 1:499 and 1:19, respectively. Mechanical inoculation ofNicotiana debneyi gave variable results with detection possible 38% of the time at dilutions of 1:499.

Potato virus X (PVX) was consistently detected by ELISA and LAT at dilutions (PVX sap: healthy sap) of 1:199 and 1:49, respectively. Mechanical inoculation ofGomphrena globosa with PVX resulted in consistent detection of the virus at a dilution of 1:499.

Key Word

Bioassay 

Resumen

El virusS de la papa (PVS), fue detectado consistentemente por la técnica serológica con conjugados enzimáticos (ELISA), y la prueba de la floculación por latex (LAT) en diluciones (Jugo con PVS: jugo libre de virus) de 1:499 y 1:19, respectivamente. La inoculación mecánica deNicotiana debneyi dio resultados variables, con una detección posible, en 38% de los casos, a diluciones de 1:499.

El virusX de la papa, fue consistentemente detectado por ELISA y LAT, a diluciones (Jugo con PVX: jugo libre de virus) de 1:199 y 1:49, respectivamente. La inoculación mecánica deGomphrena globosa con PVX dio por resultado una consistente detección del virus a una dilución de 1:499.

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Copyright information

© Springer 1986

Authors and Affiliations

  • Gary D. Franc
    • 1
  • Ernest E. Banttari
    • 2
  1. 1.Department of Plant Pathology and Weed ScienceColorado State UniversityFort Collins
  2. 2.Department of Plant PathologyUniversity of MinnesotaSt. Paul

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