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American Potato Journal

, Volume 68, Issue 6, pp 363–372 | Cite as

Insect transmission of the bacterial ring rot pathogen

  • R. D. Christie
  • A. C. Sumalde
  • J. T. Schulz
  • N. C. Gudmestad
Article

Abstract

Three species of potato-infesting insects were evaluated under laboratory and field conditions for their capability to transmitCorynebacterium sepedonicum (Spieck. & Kotth.) Skapt. & Burkh., the bacterial ring rot (BRR) pathogen of potatoes. Laboratory and field studies confirmed the vector capability of the Colorado potato beetle (CPB),Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) and the green peach aphid (GPA),Myzus persicae (Sulzer) (Homoptera: Aphididae). Both adults and larvae of the CPB proved to be vectors of the pathogen. Only adult GPA were evaluated. Bacterial transmission was most likely mechanical: analyses of the CPB mouthparts stained by the indirect immunofluorescence antibody staining (IFAS) procedure confirmed the presence of the bacterium after the beetles were exposed to infected plant tissue. Eggs, haemolymph, feces, regurgitations, and macerates of the digestive tract failed to show the presence of the bacterium. The aster leafhopper,Macrosteles fascifrons (St∮al) (Homoptera: Cicadellidae) failed to acquire and transmitCorynebacterium sepedonicum.

Additional Key Words

Green peach aphid Colorado potato beetle aster leaf-hopper indirect immunofluorescence antibody staining (IFAS) 

Compendio

Se evaluaron bajo condiciones de laboratorio y de campo tres especies de insectos que infestan a la papa para determinar su capacidad para transmitirCorynebacterium sepedonicum (Spieck. & Kotth.) Skapt. & Burkh., la bacteria patógena de la pudrición anular (BRR) de la papa. Los estudios de laboratorio y de campo confirmaron la capacidad del escarabajo de la papa de Colorado (CPB),Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) y del áfido verde del melocotonero (GPA),Myzus persicae (Sulzer) (Homoptera: Aphididae). Tanto adultos como larvas del CPB mostraron ser vectores del patógeno. Se evaluaron solamente los adultos del GPA. La transmisión de la bacteria en su mayor parte fue posiblemente mecánica: análisis de las piezas bucales del CPB teñidas mediante el método de tinción de anticuerpos por inmunofluorescencia indirecta (IFAS) confirmó la presencia de la bacteria después que los escarabajos fueron expuestos para infector los tejidos de las plantas.

No se detectó la presencia de la bacteria en huevos, hemolinfa, excrementos, regurgitaciones, y maceraciones del tracto digestivo. La cigarrita del aster,Macrosteles fascifrons (Stal) (Homoptera: Cicadellidae) fracasó en adquirir y transmitirCorynebacterium sepedonicum.

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Copyright information

© Springer 1991

Authors and Affiliations

  • R. D. Christie
    • 1
  • A. C. Sumalde
    • 3
  • J. T. Schulz
  • N. C. Gudmestad
    • 2
  1. 1.Dept. of EntomologyNorth Dakota State UniversityFargo
  2. 2.Dept. of Plant PathologyNorth Dakota State UniversityFargo
  3. 3.Dept. of EntomologyU. of Philippines at Los Baños CollegeLagunaPhilippines

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