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American Potato Journal

, Volume 53, Issue 12, pp 443–455 | Cite as

Variability in the concentration of three heat stable proteinase inhibitor proteins in potato tubers

  • C. A. Ryan
  • T. Kuo
  • G. Pearce
  • R. Kunkel
Article

Abstract

The variability of three well characterized proteinase inhibitors, Inhibitor I, molecular weight 39,000, Inhibitor II, molecular weight 21,000, and Carboxypeptidase Inhibitor, molecular weight 4,100, were determined in apical cortical tissues of individual potato tubers of the Russet Burbank variety. The three inhibitors varied within ± 20% among sixty-five tubers and cumulatively represented about 7% of the total soluble proteins. The inhibitors were highly variable among tubers of 106 clones from randomly chosen varieties. Inhibitor I varied about twelve-fold (60 to 745 μg/ml juice), and Inhibitor II varied about seven-fold (158 to 1,025 μg/ml juice). Carboxypeptidase Inhibitor varied from as low as zero (seven varieties) to over 850 μg/ml tuber juice. With 80 tubers from fourteen varieties of potatoes, a positivecorrelation was found between the concentrations of Inhibitor I and Inhibitor II and total soluble protein. Carboxypeptidase Inhibitor did not correlate well with total soluble protein. The positive correlations of Inhibitors I and II (a correlation coefficient of 0.70) with total soluble protein indicated that the proteinase inhibitors may be excellent markers for genetic studies for selecting high protein potato tuber varieties.

Keywords

Potato Tuber AMERICAN Potato Journal Total Soluble Protein Russet Burbank Potato Potato Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Resumen

La variabilidad de tres inhibidores de proteínasa bien caracterizados, Inhibidor I, 39,000 de peso molecular, Inhibidor II, 21,000 de peso molecular y un Inhibidor de carboxypeptidasa, 4100 de peso molecular, fueron determinados en tejido cortical del ápice de tubérculos individuates de papa de la variedad Russet Burbank. Los tres inhibidores variaron dentro ±20% entre sesenta y cinco tubeŕculos y acumulativamente representaron aproximadamente 7% de las proteínas solubles totales. Los inhibidores fueron muy variables entre los tubérculos de 106 clones de muestras escogidas al azar. El Inhibidor I varió alrededor de doce veces (60 a 745 μg/ml de judo) y el Inhibidor II varió aproximadamente siete veces (158 a 1025 μg/ml de jugo). El Inhibidor de carboxypeptidasa varió desde valores tan bajo como cero (siete variedades) a por encima de 850 μg/ml de jugo de tubérculos. Con 80 tubérculos de 14 variedades de papa, se encontró una correlación positiva entre las concentraciones del Inhibidor I y del Inhibidor II y la proteína soluble total. El Inhibidor de carboxypeptidasa no correlacioné bien con la proteina soluble total. Las correlaciones positivas de los inhibidores I y II (un coeficiente de correlación de 0.70) con la proteína soluble total indicó que los inhibidores de proteinasa pueden ser excelentes parámetras para los estudios de génetica en la selección de variedades de papa con alta proteína en el tubérculo.

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Copyright information

© Springer 1976

Authors and Affiliations

  • C. A. Ryan
    • 1
    • 2
  • T. Kuo
    • 1
    • 2
  • G. Pearce
    • 1
    • 2
  • R. Kunkel
    • 1
    • 2
  1. 1.Department of Agricultural Chemistry, Program in Biochemistry and Biophysics Department of HorticultureWashington State UniversityPullman
  2. 2.Department of HorticultureWashington State UniversityPullman

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