American Potato Journal

, Volume 52, Issue 4, pp 117–123 | Cite as

Evaluation of procedures for detection of pectolyticErwinia spp. on potato tubers

  • S. H. De Boer
  • A. Kelman


The effectiveness of a tuber incubation method for detection ofErwinia carotovora var.atroseptica andE. carotovora var.carotovora in potato tubers was compared with a lenticel sampling procedure. In the first method, tubers were injured by puncturing lenticels with sterile toothpicks, then wrapped in moist paper towels and polyvinylidene film, and placed in closed chambers flushed with N2. In later experiments, wrapping tubers in two layers of polyvinylidene film and incubation in air was found to be as effective as the single layer of polyvinylidene and incubation in chambers flushed with N2. Isolations were made on a selective crystal violet pectate (CVP) medium from homogenized samples of tissue removed from soft rot lesions developing around injured lenticels. In the second method, 10 lenticels/tuber were aseptically removed with a scalpel and homogenized in distilled water; the suspension was plated on CVP. The first method was less tedious and slightly more effective than the lenticel sampling method. In a preliminary survey, these methods were used to detectErwinia infestations in small samples of certified seed potato tubers from Maine, Minnesota, Montana, New York, North Dakota, and Wisconsin. PectolyticErwinia spp. were detected in at least one sample from each state except Montana. The percentage of tubers withErwinia infestations varied from 0–100% among samples. Characterization ofErwinia isolates showed that bothE. carotovora var.carotovora andE. carotovora var.atroseptica were present. PectolyticErwinia spp. on symptomless potato seed tubers may serve as inoculum sources for blackleg and soft rot diseases.


Potato Tuber AMERICAN Potato Journal Seed Potato Seed Tuber Moist Paper Towel 
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Se comparó la eficacia de un método de incubación de tubérculos con otro de muestreo de lenticelas para detectarErwinia carotovora var.atroseptica y E. carotovora var.carotovora. Para el primer método, los tubérculos fueron heridos punzando las lenticelas con mondadientes esterilizados, luego fueron envueltos en toallas de papel húmedas y en una lámina de polivinilideno (Pv.) y colocados en cámaras cerradas inundadas con N2. En los experimentos posteriores, se determinó que era tan efectivo envol ver los tubérculos en dos capas de Pv. con incubatión en aire como la capa simple de Pv. con incubación en cámaras inundadas con N2. Se hicieron aislamientos en un medio selectivo de cristal violeta pectato (CVP) de muestras homogenizadas de tejido extirpado de lesiones de podredumbre blanda en desarrollo alrededor de lenticelas heridas. En el segundo método, se extirparon asepticamente 10 lenticelas por tubérculo con un bisturí, y se homogenizaron en agua destilada; la suspensión fue sembrada en CVP. El primer método fue menos tedioso y ligeramente más efectivo que el método de muestreo de lenticelas. En una encuesta preliminar, estos métodos fueron usados para detectar infestaciones deErwinia en pequeñas muestras de semilla certificada procedente de los Estados de Maine, Minnesota, Montana, Nueva York, Nor-Dakota y Wisconsin. Se determinóErwinia spp. en por lo menos una muestra de cada Estado, exceptuando a Montana. El porcentaje de tubérculos conErwinia varió de 0–100% entre muestras. La caracterización de los aislamientos deErwinia demostraron la presencia de ambosE. carotovora var.carotovora yE. carotovora var.atroseptica. Las especies pectolíticas deErwinia en tubérculos de semilla de papa sin s’intomas pueden actuar como fuentes de inóculo para las enfermedades piema negra y podredumbre blanda.


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Copyright information

© Springer 1975

Authors and Affiliations

  • S. H. De Boer
    • 1
  • A. Kelman
    • 1
  1. 1.Department of Plant PathologyUniversity of WisconsinMadison

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