American Potato Journal

, Volume 74, Issue 5, pp 305–313 | Cite as

Potato leafroll virus detection by RT-PCR in field-collected aphids

  • Rudra P. Singh
  • J. Kurz
  • G. Boiteau
  • Lynn M. Moore


A reverse transcription polymerase chain reaction (RT-PCR) assay was applied to analyze green peach aphids (Myzus persicae Sulzer) collected from yellow-pan traps between 1988 and 1994, from New Brunswick commercial potato fields, for potato leafroll virus (PLRV). The RT-PCR technique was also used to assess the PLRV content ofMyzus persicae Sulzer,Aphis nasturtii Kaltenbach, andMacrosiphum euphorbiae Thomas caught in yellow-pan traps from selected fields in 1995. RT-PCR bands specific for PLRV were detected in all three species, indicating that they all can acquire the virus. The usefulness of the RT-PCR procedure in determining the PLRV content of aphid catches and thus assisting in disease management recommendations is discussed.

Additional Key Words

RT-PCR virus detection epidemiology virus transmission virus spread 


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Copyright information

© Springer 1997

Authors and Affiliations

  • Rudra P. Singh
    • 1
  • J. Kurz
    • 1
  • G. Boiteau
    • 1
  • Lynn M. Moore
    • 2
  1. 1.Potato Research CentreAgriculture and Agri-Food CanadaFrederictonCanada
  2. 2.New Brunswick Department of Agriculture and Rural DevelopmentFlorenceville

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