Potato leafroll virus detection by RT-PCR in field-collected aphids
- 139 Downloads
A reverse transcription polymerase chain reaction (RT-PCR) assay was applied to analyze green peach aphids (Myzus persicae Sulzer) collected from yellow-pan traps between 1988 and 1994, from New Brunswick commercial potato fields, for potato leafroll virus (PLRV). The RT-PCR technique was also used to assess the PLRV content ofMyzus persicae Sulzer,Aphis nasturtii Kaltenbach, andMacrosiphum euphorbiae Thomas caught in yellow-pan traps from selected fields in 1995. RT-PCR bands specific for PLRV were detected in all three species, indicating that they all can acquire the virus. The usefulness of the RT-PCR procedure in determining the PLRV content of aphid catches and thus assisting in disease management recommendations is discussed.
Additional Key WordsRT-PCR virus detection epidemiology virus transmission virus spread
Unable to display preview. Download preview PDF.
- Boiteau, G., R.P. Singh, R.H. Parry, and Y. Pelletier. 1988. The spread of PVY° in New Brunswick potato fields: timing and vectors. Am Potato J 65: 639–649.Google Scholar
- MacGillivray, M.E. 1981. AphidsIn: Compendium of Potato Diseases. W.J. Hooker (Ed.). The American Phytopathological Society Press, St. Paul, Minnesota. pp. 101–103.Google Scholar
- Parry, R.H. 1987. Aphid and virus management in potatoes in eastern Canada.In: Potato Pest Management in Canada, Symposium on Improving Potato Pest Protection, Fredericton, NB, Canada. Canada-New Brunswick Agri-Food Development Agreement. pp. 9–12.Google Scholar
- Radcliffe, E.B., D.W. Ragsdale, and K.L. Flanders. 1993. Management of aphids and leafhoppers.In: Potato Health Management. R.C. Rowe (Ed.). The American Phytopathological Society Press, St. Paul, Minnesota. pp. 117–126.Google Scholar