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American Potato Journal

, Volume 74, Issue 5, pp 305–313 | Cite as

Potato leafroll virus detection by RT-PCR in field-collected aphids

  • Rudra P. Singh
  • J. Kurz
  • G. Boiteau
  • Lynn M. Moore
Article

Abstract

A reverse transcription polymerase chain reaction (RT-PCR) assay was applied to analyze green peach aphids (Myzus persicae Sulzer) collected from yellow-pan traps between 1988 and 1994, from New Brunswick commercial potato fields, for potato leafroll virus (PLRV). The RT-PCR technique was also used to assess the PLRV content ofMyzus persicae Sulzer,Aphis nasturtii Kaltenbach, andMacrosiphum euphorbiae Thomas caught in yellow-pan traps from selected fields in 1995. RT-PCR bands specific for PLRV were detected in all three species, indicating that they all can acquire the virus. The usefulness of the RT-PCR procedure in determining the PLRV content of aphid catches and thus assisting in disease management recommendations is discussed.

Additional Key Words

RT-PCR virus detection epidemiology virus transmission virus spread 

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Literature Cited

  1. Bagnall, R.H. 1988. Epidemics of potato leafroll in North America and Europe linked to drought and sun spot cycles. Can J Plant Pathol 10: 193–202.CrossRefGoogle Scholar
  2. Boiteau, G., R.P. Singh, R.H. Parry, and Y. Pelletier. 1988. The spread of PVY° in New Brunswick potato fields: timing and vectors. Am Potato J 65: 639–649.Google Scholar
  3. Clarke, R.G., R.H. Converse, and M. Kojima. 1980. Enzyme-linked immunosorbent assay to detect potato leafroll virus in potato tubers and viruliferous aphids. Plant Dis 64: 43–45.CrossRefGoogle Scholar
  4. Hadidi, A., M.S. Montasser, L. Levy, R.W. Goth, R.H. Converse, M.A. Madkour, and L.J. Skrzeckowski. 1993. Detection of potato leafroll and strawberry mild yellow-edge luteoviruses by reverse transcription-polymerase chain reaction amplification. Plant Dis 77: 595–601.CrossRefGoogle Scholar
  5. Lopez-Moya, J.J., J. Cubero, D. López-Abella, and J.R. Diaz-Ruiz. 1992. Detection of cauliflower mosaic virus (CaMV) in single aphids by the polymerase chain reaction (PCR). J Virol Meth 37: 129–138.CrossRefGoogle Scholar
  6. MacGillivray, M.E. 1972. The sexuality ofMyzus persicae (Sulzer), the green peach aphid, in New Brunswick. Can J Zool 50: 469–471.CrossRefGoogle Scholar
  7. MacGillivray, M.E. 1981. AphidsIn: Compendium of Potato Diseases. W.J. Hooker (Ed.). The American Phytopathological Society Press, St. Paul, Minnesota. pp. 101–103.Google Scholar
  8. Parry, R.H. 1987. Aphid and virus management in potatoes in eastern Canada.In: Potato Pest Management in Canada, Symposium on Improving Potato Pest Protection, Fredericton, NB, Canada. Canada-New Brunswick Agri-Food Development Agreement. pp. 9–12.Google Scholar
  9. Radcliffe, E.B., D.W. Ragsdale, and K.L. Flanders. 1993. Management of aphids and leafhoppers.In: Potato Health Management. R.C. Rowe (Ed.). The American Phytopathological Society Press, St. Paul, Minnesota. pp. 117–126.Google Scholar
  10. Singh, M. and R.P. Singh. 1995. Digoxigenin-labelled cDNA probes for the detection of potato virus Y in dormant tubers. J Virol Meth 52: 133–143.CrossRefGoogle Scholar
  11. Singh, M. and R.P. Singh. 1996. Factors affecting detection of PVY in dormant tubers by reverse transcription polymerase chain reaction and nucleic acid spot hybridization. J Virol Meth 60: 47–57.CrossRefGoogle Scholar
  12. Singh, R.P., J. Kurz, G. Boiteau, and G. Bernard. 1995. Detection of potato leafroll virus in single aphids by the reverse transcription polymerase chain reaction and its potential epidemiological application. J Virol Meth 55: 133–143.CrossRefGoogle Scholar
  13. Singh, R.P., J. Kurz, and G. Boiteau. 1996. Detection of stylet-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. J Virol Meth 59: 189–196.CrossRefGoogle Scholar
  14. Smith, O.P., V.D. Damsteegt, C.J. Keller, and R.J. Beck. 1993. Detection of potato leafroll virus in leaf and aphid extracts by dot-blot hybridization. Plant Dis 77: 1098–1102.CrossRefGoogle Scholar
  15. Spiegel, S. and R.R. Martin. 1993. Improved detection of potato leafroll virus in dormant potato tubers and microtubers by the polymerase chain reaction and ELISA. Ann Appl Biol 122: 493–500.CrossRefGoogle Scholar
  16. Tamada, T. and B.D. Harrison. 1981. Quantitative studies on the uptake and retention of potato leafroll virus by aphids in laboratory and field conditions. Ann Appl Biol 98: 261–276.CrossRefGoogle Scholar

Copyright information

© Springer 1997

Authors and Affiliations

  • Rudra P. Singh
    • 1
  • J. Kurz
    • 1
  • G. Boiteau
    • 1
  • Lynn M. Moore
    • 2
  1. 1.Potato Research CentreAgriculture and Agri-Food CanadaFrederictonCanada
  2. 2.New Brunswick Department of Agriculture and Rural DevelopmentFlorenceville

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