Effect of NH4-non the pigment content ofLaminaria japonica

  • Dong Liangfeng
  • Lin Guangheng
  • Wu Chaoyuan


Experiments withLaminaria japonica were conducted in Meidao Bay and Pier Bay, Qingdao, China, Nitrogen-starved plants were fertilized intermittently with 7.1 mM NH4−N solution for 1 hour at 3 day intervals, after which chlorophyll a, fucoxanthin, chlorophyll c and β-carotene contents were analyzed. Photosynthetic and growth rates of plants and nitrogen content of seawater were determined. their contents of chlorophyll a, fucoxanthin, chlorophyll c and β-carotene were 1.65, 0.67, 0.33 and 0.06−2 respectively, or 2.46, 2.03, 1.86 and 1.81 times those of the controls. The ratio of fucoxanthin to chlorophyll a in nitrogen-enriched plants was lower than that of the controls. Normal growth rate of the plants (2 cm·day−1 in length) were reached when the chlorophyll a content exceeded 0.1 mg·g−1 fresh wt., indicating that chlorophyll a content can serve as an indicator of normal growth.

Experimental results show that seawater is regarded as fertile for the normal growth ofLaminaria if the total inorganic nitrogen (including ammonium salt, nitrate and nitrite) content in seawater is about 2 μm, and infertile if less than 1 μm, in which case fertilizer should be applied.


Chlorophyll Pier Photosynthetic Rate Pigment Content Brown Seaweed 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alberte, R. S., A. L. Fridman, D. L. Gustafson, M. S. Rudnick and H. Lyman, 1981. Light-harvesting systems of brown algae and diatoms. Isolation and characterization of chlorophyll a/c and chlorophyll a/fucoxanthin pigment-protein complexes.Biochem. Biophys. Acta 635: 304–316.CrossRefGoogle Scholar
  2. Barrett, J. and J. M. Anderson, 1980. The P—700—chlorophyll a-protein complex and two major light-harvesting complexes ofAcrocarpia paniculata and other brown seaweeds.Biochem. Biophys. Acta 590: 309–323.CrossRefGoogle Scholar
  3. Davison, I. R., M. Andrews and W. D. P. Stewart, 1984. Regulation of growth inLaminaria digitata: use of in-vivo nitrate reductase activities as an indicator of nitrogen limitation in field populations ofLaminaria spp.Mar. Biol. 84: 207–217.CrossRefGoogle Scholar
  4. Dring, M. J., 1982. Photosynthesis in the sea.In: The Biology of Marine Plants (Ed. by M. J. Dring). Edward Arnold Limited, London, Great Britain. pp. 47–55.Google Scholar
  5. Epply, R. W., 1978. Nitrate uptake,In: Handbook of phycological methods. Physiological and Biochemical Methods. (Ed. by Hellebust, J. A. and J. S. Craigie). Cambridge University Press, Cambridge, Great Britain, pp. 401–411.Google Scholar
  6. Gao Fengming, Zhang Shuhua, Wang Xienyuan, Liang Zueping, Xiu Ling and Wu Baoshan, 1980. Studies on a NaBrO method for the determination of ammonia in seawater.Transac. Oceanol. Limnol. 4, 41–46. (in Chinese).Google Scholar
  7. Lapointe, B. E. and J. H. Ryther, 1979. The effects of nitrogen and seawater flow rate on the growth and biochemical composition ofGracilaria follifera var. angustissima in mass outdoor cultures.Bot. Mar. 22. 529–537.CrossRefGoogle Scholar
  8. Lapointe, B. E. 1981. The effect of light and nitrogen on growth, pigment content, and biochemical composition ofGracilaria folliifera v. angustissima.J. Phycol. 17: 90–95.CrossRefGoogle Scholar
  9. Mann, K. H., 1972. Ecological energetics of the seaweed zone in a marine bay on the Atlantic coast of Canada. I. Zonation and biomass of seaweed.Mar. Biol. 14: 199–209.Google Scholar
  10. Niell, F. X., 1976. C∶N ratio in some marine macrophytes and its possible ecological significance.Bot. Mar. 19: 347–350.CrossRefGoogle Scholar
  11. Pan Ruzhi and Dong Yude, 1979. Plant Physiology. People Education Press, Beijing, China. p. 90 (in Chinese).Google Scholar
  12. Peyriere, M., L. Caron and H. Jupin, 1984. Pigment complexes and energy transfers in brown algae.Photosynthetica 18: 184–191.Google Scholar
  13. Ryther, J. H., H. Corwin, T. A. DeBusk and L. D. Williams, 1981. Nitrogen uptake and storage by the red algaGracilaria tikvahiae (McLachlan, 1979).Aquacult. 26: 107–115.CrossRefGoogle Scholar
  14. Seely, G. R., M. J. Duncan and W. E. Vidaver, 1972. Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide.Mar. Biol. 12: 184–188.CrossRefGoogle Scholar
  15. Wu Chaoyuan, 1962a. Relationship between the growth and development of sporophyte and environment.In. Manual of Haidai (Laminaria japonica Aresch) Cultivation (Ed. C. K. Tseng and C. Y. Wu). Science Press, Beijing, China. pp. 42–56. (in Chinese)Google Scholar
  16. Wu Chaoyuan, 1962b. Nutrient substance transportation and accumulation.In.Manual of Haidai (Laminaria japonica Aresch). Cultivation (Ed. C. K. Tseng and C. Y. Wu). Science Press, Beijing, China. p. 80 (in Chinese)Google Scholar
  17. Wu Chaoyuan, 1962c. Relationship between the growth and development of sporophyte and environment.In: Manual of Haidai (Laminaria japonica Aresch.) Cultivation (Ed. C. K. Tseng and C. Y. Wu). Science Press, Beijing, China. pp. 68–69. (in Chinese)Google Scholar
  18. Wu Chaoyuan Wen Zongcun, Peng Zuosheng and Zhang Jingpu. 1984. A preliminary comparative study of the productivity of three economic seaweed.Chin. J. Oceanol. Limnol. 2: 97–101.CrossRefGoogle Scholar
  19. Yao Nanyu and Li Jianzhi, 1981. Studies on the photosynthesis ofLaminaria japonica Aresch.Plant Physiol. Communica. 4: 18–21. (in Chinese)Google Scholar

Copyright information

© Science Press 1990

Authors and Affiliations

  • Dong Liangfeng
    • 1
  • Lin Guangheng
    • 1
  • Wu Chaoyuan
    • 1
  1. 1.Institute of OceanologyAcademia SinicaQingdao

Personalised recommendations