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Examination of the rescue effects of folic acid on derangement of the tubo-ovarian ultrastructural architecture caused by methotrexate

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Abstract

The purpose of this examination was to observe the effects of folic acid (FA) on methotrexate (MTX)-induced derangements in the fallopian tubes. Investigators in this study sought to explore whether MTX-induced dysfunction in the fallopian tubes would be lessened by the addition of FA to MTX treatment. For this study, 18 albino Wistar rats were randomly divided into 6 groups, each of which comprised 3 rats; 0.1 mg/kg FA, 1 mg/kg MTX + 0.1 mg/kg FA, 5 mg/kg MTX + 0.1 mg/kg FA, 1 mg/kg MTX, and 5 mg/kg MTX were given to groups 2, 3, 4, 5, and 6, respectively; group 1 was the control group. After MTX injection, fallopian tube samples from all groups were prepared for examination under electron microscopy. The findings observed in groups 1 and 2 were similar. The level of cellular destruction was greater with the higher doses of MTX without FA; in particular, loss of cilia in the epithelium was prominent in groups 5 and 6. However, there was less cellular destruction observed in groups 3 and 4 than in groups 5 and 6. As a result, the addition of FA should not be overlooked, even when a single-dose MTX regimen is chosen for the treatment of patients with unruptured ectopic pregnancy

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Bilkent-3 C:4 No:7, 06800, Bilkent-Ankara, Turkey

    Merih Bayram (Assoc Prof)

  2. Department of Histology and Embryology, Faculty of Medicine Gazi University, Ankara, Turkey

    Candan Ozogul (Prof)

  3. Department of Pharmacology, Faculty of Medicine Gazi University, Ankara, Turkey

    Z. Sevim Ercan (Prof)

  4. Department of Pharmacology, Faculty of Medicine Gazi University, Ankara, Turkey

    Ergin Dilekoz (Research Fellow)

  5. Department of Obstetrics and Gynecology, Kecioren Hospital, Ankara, Turkey

    Canan Soyer MD

  6. Department of General Surgery, Gazi University, Ankara, Turkey

    Orhan Bayram (Prof)

Authors
  1. Merih Bayram
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  2. Candan Ozogul
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  3. Z. Sevim Ercan
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  4. Ergin Dilekoz
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  5. Canan Soyer MD
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  6. Orhan Bayram
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Bayram, M., Ozogul, C., Ercan, Z.S. et al. Examination of the rescue effects of folic acid on derangement of the tubo-ovarian ultrastructural architecture caused by methotrexate. Adv Therapy 23, 772–777 (2006). https://doi.org/10.1007/BF02850317

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  • DOI: https://doi.org/10.1007/BF02850317

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