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Evaluation of thep53 tumor suppressor gene mutation in normal rat salivary gland tissue after radioiodine application: An experimental study

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Abstract

In this experimental study, investigators exploredp53 tumor suppressor gene mutation induced by low and high doses of iodine-131 sodium iodide (I-131) in salivary gland tissue in rats. Group 1 consisted of 10 rats; low and high I-131 doses were applied at a 1-wk interval. First, low doses of I-131 were injected. (The net injected dose was 47.5±9.2 μCi.) After 1 wk, high doses of I-131 were also injected. (The net injected dose was 1007.2±53 μCi.) Group 2 consisted of 5 rats, and only a low I-131 dose was applied. (The net injected dose was 52.7±5.5 μCi.) The Control Group consisted of 5 rats that did not receive I-131. Thyroidal I-131 uptakes were calculated for Groups 1 and 2 with the use of a gamma camera after 24 h of injections. Immediately after uptake was calculated, salivary glands were resected in all groups and DNA was extracted for genotyping. Genomic DNA of thep53 gene exon 5 was examined by polymerase chain reaction single-strand conformational polymorphism. In Group 1, thyroidal I-131 uptakes were calculated as 12.45%±4.14% and 9.66%±6.73% after low-dose and high-dose I-131 applications, respectively. In Group 2, thyroidal I-131 uptake was calculated as 13.12%±3.04%. In Group 1,p53 gene abnormality was seen in the salivary gland of only 1 of the rats. Double and single-strand gene profiles showed that both alleles of this rat have a mutated single-strand conformational polymorphism profile of point mutation in thep53 gene exon 5. This rat received the highest low dose and the second highest total dose of I-131; its thyroidal uptakes were the second highest. In the other rats in Group 1, and in Group 2 and the Control Group,p53 gene abnormalities were not observed. In Groups 1 and 2, a significant relationship could not be discerned between thyroidal uptake of I-131 andp53 gene mutation in the salivary gland. No significant relationship was observed between thyroidal uptake alterations andp53 gene mutations in salivary glands in Group 1. A point mutation in thep53 gene exon 5 that was seen in only 1 of the rats in Group 1 seems related to the high-dose application of I-131, although coincidental occurrences could not be excluded. We believe that this topic is open to additional in vivo studies.

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Authors and Affiliations

  1. Department of Nuclear Medicine, Cumhuriyet University School of Medicine, P.K. 848, Campus, 58140, Sivas, Turkey

    Bulent Turgut MD (Assistant Professor) & Taner Erselcan MD (Associate Professor)

  2. Department of Medical Genetics, Cumhuriyet University School of Medicine, Sivas, Turkey

    Ozturk Ozdemir PhD (Associate Professor)

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  1. Bulent Turgut MD
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  2. Ozturk Ozdemir PhD
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  3. Taner Erselcan MD
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Turgut, B., Ozdemir, O. & Erselcan, T. Evaluation of thep53 tumor suppressor gene mutation in normal rat salivary gland tissue after radioiodine application: An experimental study. Adv Therapy 23, 456–468 (2006). https://doi.org/10.1007/BF02850167

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