American Potato Journal

, Volume 72, Issue 11, pp 689–700 | Cite as

Yield of embryos from sequential anther culture

  • L. Y. Shen
  • R. E. Veilleux


Experiments were conducted on sequential anther culture,i.e., returning anthers into medium following the usual harvest of embryos six weeks after culture initiation. Three diploid interspecific hybrid potato clones were used. Additional embryos were produced after the first harvest, although the quality and regeneration frequency of such embryos were generally poorer than for first harvest embryos. In addition, embryos from the second harvest required more time to regenerate into plants. Significantly more embryos were produced when anthers were returned to the same medium (16 per flask) compared to fresh medium (4 per flask) in sequential anther culture. Regression analysis showed that embryo yields at the first and second harvests were significantly correlated (P<0.01). Poor anther culture yield at the first harvest was not caused by a longer lag period. Highly responsive cultures continued to yield embryos even 12 weeks after culture initiation.

Additional Key Words

Androgenesis conditioned medium embryo regeneration secondary embryogenesis Solanum andigena Solatium bertkaultii Solatium chacoense Solatium microdontum Solanum phureja 


Se condujeron experimentos sobre el cultivo sucesivo de anteras, es decir, regresando a las anteras al medio después de la cosecha normal de embriones seis semanas después de iniciado el cultivo. Se utilizaron tres clones híbridos interespecíficos diploides de papa. Después de la primera cosecha se produjeron embriones adicionales, aunque la calidad y frecuencia de regeneratión de los mismos fueron generalmente más pobres que las de los embriones de la primera cosecha. Además, los embriones de la segunda cosecha requirieon más tiempo para convertirse en plantas regeneradas. Significativamente, en el cultivo sucesivo de anteras se produjeron más embriones cuando las anteras fueron nuevamente cultivadas en el mismo medio (16 por frasco) en comparación con las cultivadas en medio fresco (cuatro por frasco). El análisis de regresión mostró que los rendimientos en embriones en la primera y segunda cosechas estuvieron significativamente correlacionados (P<0.01). El bajo rendimiento del cultivo de anteras en la primera cosecha no fue causado por un periodo más prolongado de espéra. Cultivos altamente rendidores continuaron produciendo embriones incluso 12 semanas después de haberse iniciado.


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Copyright information

© Springer 1995

Authors and Affiliations

  • L. Y. Shen
    • 1
  • R. E. Veilleux
    • 1
  1. 1.Department of HorticultureVirginia Polytechnic Institute and State UniversityBlacksburg

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