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American Potato Journal

, Volume 74, Issue 1, pp 15–21 | Cite as

Diploid and tetraploidSolanum chacoense genotypes that synthesize leptine glycoalkaloids and deter feeding by Colorado potato beetle

  • L. L. Sanford
  • R. S. Kobayashi
  • K. L. Deahl
  • S. L. Sinden
Article

Abstract

A selection (8380-1) fromSolatium chacoense Bitter (2n=2x=24) accession PI 458310 that synthesizes the leptine glycoalkaloids was compared in growth chambers with tetraploid (2n=4x=48) genotypes derived from tissue culture of 8380-1 leaf expiants for plant growth habit, leaf glycoalkaloid content, and effect on the development of Colorado potato beetle [Leptinotarsa decemlineata (Say)] larvae.

The plants of the 4x regenerant genotypes were more vigorous with larger, more oval shaped leaflets than 8380-1 plants. The leaf concentrations of leptines and total glycoalkaloids were significantly lower (about 34%) in the 4x genotypes than in 8380-1. The proportion of leptines in the total glycoalkaloid content was nearly the same (about 80%) in both ploidy groups. In leaf-disk feeding tests, the development of Colorado potato beetle neonate larvae was not significantly different for the 2x and 4x genotypes. Both groups significantly slowed development compared with development on cv. Kennebec leaf disks.

The 8380-1 selection and a group of 4x 8380-1 regenerant genotypes are maintained in the Vegetable Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705 and are available for distribution.

Additional Key Words

Leptinotarsa decemlineata resistance tuberosum 

Compendio

Una selección (8380–1) deSolanum chacoense Bitter (2n=2x=24), entrada PI 458310, que sintetiza los glicoalcaloides de la leptina fue comparada en cámaras de crecimiento con genotipos tetraploides (2n=24=48) derivados del cultivo de tejidos de regenerantes de las hojas de la selección 8380–1 en cuanto a sus hábitos de crecimiento, el contenido de glicoalcaloides en las hojas y su efecto en el desarrollo de las larvas del escarabajo de la papa de Colorado (Leptinotarsa decemlineata Say). Las plantas de los regenerantes de genotipo 4x fueron más vigorosas, con folíolos más grandes y más ovalados, que las plantas 8380–1. Las concentraciones de leptinas y el total de glicoalcaloides de la hoja fueron significativamente menores (alrededor del 34%) en los genotipos 4x que en la selección 8380–1. La proporción de leptinas dentro del contenido total de glicoalcaloides fue casi la misma (aproximadamente 80%) en ambos grupos de ploidia. En las pruebas de alimentatión con discos foliares, el desarrollo de las larvas neonatas del escarabajo de Colorado no tuvo una diferencia significativa para los genotipos 2x y 4x. Ambos grupos tuvieron un desarrollo más lento en comparación con el desarrollo en los discos foliares del cv. Kennebec. La seleccion 8380–1 y un grupo de regenerantes 8380–1 de genotipo 4x son mantenidos en la Laboratorio de Plantas, Centro de Investigatión Agrícola de Beltsville, Beltsville, MD 20705, y están disponibles para su distribución.

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Copyright information

© Springer 1997

Authors and Affiliations

  • L. L. Sanford
    • 1
  • R. S. Kobayashi
    • 1
  • K. L. Deahl
    • 1
  • S. L. Sinden
    • 1
  1. 1.Agricultural Research Service, Plant Science Institute, Vegetable LaboratoryU.S. Department of AgricultureBeltsville

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