Advertisement

American Potato Journal

, Volume 70, Issue 6, pp 497–503 | Cite as

Detection of potato leafroll virus by visual inspection, direct tissue blotting and ELISA techniques

  • J. L. Whitworth
  • R. G. Samson
  • T. C. Allen
  • A. R. Mosley
Article

Abstract

Greenhouse-grown Russet Burbank and Russet Norkotah plants were tested for potato leafroll virus (PLRV) by visual examination, enzyme-linked immunosorbent assay (ELISA) and direct tissue-blotting assay (DTBA). Visibly infected and healthy plants were obtained from excised eyes of tubers submitted for winter certification tests. Each plant was sectioned into leaf, petiole, stem, root, seed piece, and tuber portions. Tissues were blotted on a nitrocellulose membrane for DTBA and then homogenized for use in a double-antibody sandwich ELISA system. Agreement between the two serological detection methods and with visual readings was high for petioles and stems, but lower for leaf, tuber and root tissues. Comparison of DTBA with ELISA and with visual plant symptoms suggest that DTBA can be used with the same accuracy as ELISA for detecting PLRV in stems and petioles

Additional Key Words

Virus detection tissue sampling immunosorbent assay PLRV 

Compendio

Plantas de “Russet Burbank” y “Russet Norkotah” producidas en invernadero fueron examinadas para detectar el virus del enrollamiento de la hoja de la papa (PLRV) por medio de un examen visual, de la prueba de conjugados enzimáticos (ELISA), y el tratamiento directode tejidos en placas de nitrocelulosa (DTBA). Plantas visiblemente infectadas y sanas fueron obtenidas de ojos extirpados de tubérculos entregados para exámenes de certificación de invierno. Cada planta era seccionada en hoja, peciolo, tallo, raíz, sección de semilla, y porciones de tubérculo. Los tejidos fueron teñidos en una membrana de nitrocelulosa para la DTBA y después homogenizados para usarlos en un sistema ELISA tipo sandwich de doble anticuerpo. La concordancia entre los dos métodos serológicos de detección y con las lecturas visuales fue alta para petiolos y tallos, pero menor para la hoja, tubérculo, y tejidos de la raíz. La comparación de la DTBA con ELISA y con los síntomas visuales de la planta sugiere que la DTBA puede usarse con la misma exactitud que ELISA para detectar PLRV én tallos y peciolos.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Clark, M.F. and A.N. Adams. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483.PubMedCrossRefGoogle Scholar
  2. 2.
    Flanders, K.L., D.W. Ragsdale and E.B. Radcliffe. 1990. Use of enzyme-linked immunosorbent assay to detect potato leafroll virus in field grown potato, cv. Russet Burbank. Am Potato J 67:589–602.Google Scholar
  3. 3.
    Harrison, B.D. 1984. Potato leafroll virus. CMI/AAB descriptions of plant viruses. No. 291. 6 pgs.Google Scholar
  4. 4.
    Lin, N.S., Y.H. Hsu and H.T. Hsu. 1990. Immunological detection of plant viruses and a mycoplasma-like organism by direct tissue blotting on nitrocellulose membranes. Phytopathology 80:824–828.CrossRefGoogle Scholar
  5. 5.
    Samson, R.G., T.C. Allen and J.L. Whitworth. 1993. Evaluation of direct tissue blotting to detect potato viruses. Am Potato J 70:257–265.Google Scholar

Copyright information

© Springer 1993

Authors and Affiliations

  • J. L. Whitworth
    • 1
  • R. G. Samson
    • 2
  • T. C. Allen
    • 2
  • A. R. Mosley
    • 1
  1. 1.Dept. of Crop and Soil ScienceOregon State Univ.Corvallis
  2. 2.Dept. of Botany and Plant PathologyOregon State Univ.Corvallis

Personalised recommendations