American Potato Journal

, Volume 71, Issue 3, pp 175–183 | Cite as

Consecutive elisa screening with monoclonal antibodies to detect potato virus YN

  • J. G. McDonald
  • G. T. Kristjansson
  • R. P. Singh
  • P. J. Ellis
  • W. B. McNab


In a 1992 survey of potato fields in Canada for the tobacco veinal necrosis strain of potato virus Y (PVYN), initial enzyme-linked immunosorbent assay (ELISA) screening with one monoclonal antibody (MAb 295.5) followed by a second ELISA screening with a panel of several MAbs (4E7, Bioreba, 1F5 and 295.5) was assessed as a means of rapidly identifying positive samples prior to confirmation by bioassay. MAbs 4E7 and Bioreba were used in double antibody sandwich (DAS) ELISA, while MAbs 1F5 and 295.5 were used in triple antibody sandwich (TAS) ELISA. In the second ELISA, MAbs 4E7, 1F5 and Bioreba all enhanced test specificity relative to the retest with MAb 295.5. All the MAbs were found to react, to some degree, with isolates of the common strain of PVY (PVYO), but MAb 4E7 was the most specific widi no cross-reaction being observed at, or above, the signal to noise (S:N) threshold of 4:1. However, this MAb was also the least sensitive. At the S:N threshold of 4:1, its sensitivity was 68%, compared to a sensitivity of close to 100% for the other MAbs

Additional Key Words

PVY Solanum tuberosum L. survey eradication bioassay 


En una encuesta de campos de papa en Canadá, en 1992, sobre la variante del virus Y de la papa (PVYN) causante de la necrosis de las nervaduras del tabaco, la evaluación inicial utilizando ELISA con un anticuerpo monoclonal (MAb 295.5) seguida por una segunda evaluación con ELISA con un panel de varios MAbs (4E7, Bioreba, 1F5 y 295.5) fue encontrada como un medio de identificación rápida de muestras positivas antes de ser confirmadas por bioensayos. Los MAbs 4E7 y Bioreba fueron utilizados en ELISA tipo sandwich de doble anticuerpo (DAS), mientras que MAbs 1F5 y 295.5 fueron utilizados en ELISA tipo sandwich de triple anticuerpo (TAS). En la segunda ELISA, MAbs 4E7, 1F5 y Bioreba mejoraron la especificidad de la prueba relativa a la repetición de la prueba con MAb 295.5. Se encontró que todos los MAbs reaccionaron en cierto grado con los aislamientos de la variante común del PVY (PVYO), pero MAb 4E7 fue el más específico, sin haberse observado reactión cruzada al nivel o por encima de una relación señal:ruido (S:R) límite de 4:1. Sin embargo, este MAb fue también el menos sensible. A la S:R límite de 4:1, su sensibilidad fue de 68%, en comparación a la sensibilidad cercana al 100% para los otros MAbs


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. 1.
    Ellis, P. J., G. Bowler, D.J. MacKenzie and R. Stace-Smith. 1992. Monoclonal antibodies for detection and identification of strains of potato virus Y in Canada and the United States. Can J Plant Pathol 14: 241 (Abst).Google Scholar
  2. 2.
    Gugerli, P. and P. Fries. 1985. Characterization of monoclonal antibodies to potato virus Y and their use for virus detection. J Gen Virol 64: 2471–2477.CrossRefGoogle Scholar
  3. 3.
    Hammond, J. 1992. Potyvirus serology, sequences and biology.In: Barnett, O.W. (ed.) Potyvirus Taxonomy. Springer, Wein New York, pp. 123–138 (Arch Virol [Suppl]5).Google Scholar
  4. 4.
    Jordan, R. 1992. Potyviruses, monoclonal antibodies and antigenic sites.In: Barnett, O.W. (ed.) Potyvirus Taxonomy. Springer, Wein, New York, pp. 81–95 (Arch Virol [Suppl]5).Google Scholar
  5. 5.
    McDonald, J.G. 1987. Comparative levels of potato viruses S and Y infection of microplants and tuber-propagated plants in the field. Am Potato J 64:517–521.Google Scholar
  6. 6.
    McDonald, J.G. and G. Kristjansson. 1993. Properties of strains of potato virus YN in North America. Plant Disease 77: 87–89.CrossRefGoogle Scholar
  7. 7.
    McDonald, J.G., I.A. MacLatchy, N.W. Sheidow, M.C. Watson and R.D. Reeleder. 1991. Tobacco veinal necrosis strain of potato virus Y (PVYN). Factsheet No. 90-001. O.M.A.F.Google Scholar
  8. 8.
    Ohshima, K., T. Hataya, T. Sano, A. K. Inoue and E. Shikata, 1991. Comparison of biological properties, serological characteristics and amino acid sequence of coat protein between potato virus Y ordinary strain and necrotic strain. Ann Phytopath Soc Japan 57: 615–622.Google Scholar
  9. 9.
    Rose, D.G. and A.I. Hubbard. 1986. Production of monoclonal antibodies for the detection of potato virus Y. Ann Appl Biol 109: 317–321.CrossRefGoogle Scholar
  10. 10.
    Rose, D.G., S. McCarra and D.H. Mitchell. 1987. Diagnosis of potato virus YN: a comparison between polyclonal and monoclonal antibodies and a biological assay. Plant Pathol 36: 95–99.CrossRefGoogle Scholar
  11. 11.
    Singh, R.P., A. Boucher, T.H. Sommerville and A.K. Dhar. 1993. Selection of a monoclonal antibody to detect PVYN in ELISA and in dot-immunobinding assays. Can J Plant Pathol 15: (in press).Google Scholar
  12. 12.
    Singh, R.P. 1992. Incidence of the tobacco veinal necrotic strain of potato virus Y (PVYN) in Canada in 1990 and 1991 and the scientific basis for the eradication of the disease. Can PI Dis Surv 72:113–119.Google Scholar
  13. 13.
    Van der Vlugt, R.A.A., J. Leunissen and R. Goldbach. 1993. Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions. Arch Virol 131:361–375.PubMedCrossRefGoogle Scholar
  14. 14.
    Young, D.A., H.T. Davies and G.R. Johnson. 1979. Jemseg: A new early high-yielding potato variety with high resistance to virus Y and immunity to virus X. Am Potato J 56:325–328.Google Scholar

Copyright information

© Springer 1994

Authors and Affiliations

  • J. G. McDonald
    • 1
  • G. T. Kristjansson
    • 1
  • R. P. Singh
    • 2
  • P. J. Ellis
    • 3
  • W. B. McNab
    • 1
  1. 1.Agriculture CanadaNepean
  2. 2.Agriculture Canada Research StationFredericton
  3. 3.Agriculture Canada Research StationVancouver

Personalised recommendations