Chinese Journal of Oceanology and Limnology

, Volume 20, Issue 3, pp 248–255 | Cite as

Physiological inhibitory effect of ocs in arachidonic acid-richParietochloris incisa (trebouxiophyceae, chlorophyta)

  • Liu Jian-guo
  • Zhang Cheng-wu
  • Zvi Cohen
  • Amos Richmond


Parietochloris incisa is an arachidonic acid-rich snow green alga. The main physiological profiles, such as ash free dry weight (AFDW), chlorophyll, carotenoid, protein and total fatty acids (TFA), in this alga exposed to old culture supernatant (OCS) at the decline phase or its crude ethyl acetate extracts (CEAE) were investigated by using tubular photobioreactors of different diameters. Results showed that both OCS and CEAE had strong inhibitory effect on the above physiological parameters. The longer the culture was exposed to OCS and the more CEAE were added into the algal culture, the more the above physiological properties were inhibited. Arachidonic acid (AA), the dominant component of fatty acids in this alga, was also seriously inhibited with respect to total TFA, AFDW of cell mass, or culture volume, due to a probable reduction of enzymes activities catalyzing chain elongation from C18; 1ω9 to AA. These results incontestably evidenced that some CEAE dissolving substances existing in OCS. like auto-inhibitors, inhibitedP. incisa growth through feedback. Hence, any efficient removal of auto-inhibitors from algal culture to decrease their bioactivity could be good for maximal production of desired products like AA.

Key words

arachidonic acid Parietochloris incisa growth auto-inhibitor 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arnon, D. I., 1949. Copper enzymes in isolated chloroplasts; polyphenol oxidase inBeta vulgaris.Plant Physiol. 24: 1–15.Google Scholar
  2. Davies, B. H., 1976. Carotenoids.In: Goodwin, T. W., eds., The Biochemistry of Carotenoids, Academic Press, N. Y., p. 38–165.Google Scholar
  3. Imada, N., Kobayashi, K., Tahara, K., et al., 1991. Production of an autoinhibitor bySkeletonema costatum and its effect on the growth of other phytoplankton.Nippon Suisan Gakkaishi 57(12): 2285–2290.Google Scholar
  4. Imada, N., Kobayashi, K., Isomura, K. et al., 1992. Isolation and identification of an autoinhibitor produced bySkeletonema costatum.Nippon Suisan Gakkaishi 58(8): 1687–1692.Google Scholar
  5. Liu, J. G., Cohen, Z., Richmond, A., 2002. Fatty Acids Profile in a High Cell Density Culture of Arachidonic Acid-RichParietochloris incisa, (Trebouxiophyceae, Chlorophyta) Exposed to High PFD.Chin. J. Oceanol. Limnol. 20(2): 149–156.CrossRefGoogle Scholar
  6. Mast, S. O., Pace, D. M., 1938. The effect of substances produced byChilomonas paramecium on the rate of reproduction.Physiol. Zool. XI (4): 359–382.Google Scholar
  7. McCracken, M. D., Middaugh, R. E., Middaugh, R. S., 1980. A chemical characterization of a algal inhibitor obtained from Chlamydomonas.Hydrobiologia 70: 271–279.CrossRefGoogle Scholar
  8. Pratt, R., 1942. Studies onChlorella vulgaris. V. Some properties of the growth—inhibitor formed by Chlorella cells.Amer. Botany 29: 142–148.CrossRefGoogle Scholar
  9. Pratt, R., 1944. Studies onChlorella vulgaris. IX. Influence on growth of Chlorella of continuous removal of chlorellin from the culture solution.Amer. Botany 31: 418–421.CrossRefGoogle Scholar
  10. Pratt, R., Fong, J., 1940. Studies onChlorella vulgaris. II. Further evidence that Chlorella cell forms a growth-in-hibiting substance.Amer. Botany 27: 431–436.CrossRefGoogle Scholar
  11. Pratt, R., Oneto, J. F., Pratt, J., 1945. Studies onChlorella vulgaris. X. Influence of the age of the culture on the accumulation of the chlorellin.Amer. Botany 32: 405–408.CrossRefGoogle Scholar
  12. Sun, Y. N., Yin, M. Y., Liu, J. G., 2001. Auto-signals inHaematococcus pluvialis.Transaction of Oceanol. and Limnol. 3: 22–28.Google Scholar
  13. Watanabe, S., Hirabayashi, S., Bossiba, S. et al., 1996.Parietochloris incisa comb. Nov. (Trebouxiophyceae, Chlorophyta).Phycol. Res. 44(2): 107–108.CrossRefGoogle Scholar

Copyright information

© Science Press 2002

Authors and Affiliations

  • Liu Jian-guo
    • 1
  • Zhang Cheng-wu
    • 2
  • Zvi Cohen
    • 3
  • Amos Richmond
    • 3
  1. 1.Research and Development Center of Marine BiotechnologyInstitute of Oceanology Chinese Academy of SciencesQingdaoChina
  2. 2.Division of life SciencesNanjing Normal UniversityNanjingChina
  3. 3.Microalgal BiotechnologyThe Jacob Bloustein Institute for Desert Research Ben-Gurion University of NegevSede Boker CampusIsrael

Personalised recommendations