Chinese Journal of Oceanology and Limnology

, Volume 10, Issue 3, pp 268–275 | Cite as

Explant exenisation for tissue culture in marine macroalgae

  • Liu Xuewu
  • Bernard Kloareg


Unialgal explants fromLaminaria digitata, and from a variety of red algae, were obtained by hand removing the visible epiphytes, and stirring the tissue in the presence of glass beads. Two antibiotic mixtures were found to be efficient in removing the contaminating fungi and bacteria from the algae. The procedure proved suitable as a primary step in the tissue culture of the investigated species.

Key words

unialgal axenic antibiotics disinfectants callus regeneration 


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Copyright information

© Science Press 1992

Authors and Affiliations

  • Liu Xuewu
    • 1
  • Bernard Kloareg
    • 1
  1. 1.Centre d'Etudes Oceanologiques et de Biologie MarineCNRS UPR 4601 et Universite Paris VIRoscoff CedexFrance

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