Chinese Journal of Oceanology and Limnology

, Volume 17, Issue 4, pp 350–358 | Cite as

Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri

  • Yang Hui-ping
  • Guo Xi-ming
  • Chen Zai-zhong
  • Wang Yong-ping


Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L) when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and 6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%, and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.

Key words

scallopChalamys farreri tetraploid Cytochalasin B mitosis I 


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Copyright information

© Science Press 1999

Authors and Affiliations

  • Yang Hui-ping
    • 1
  • Guo Xi-ming
    • 1
    • 2
  • Chen Zai-zhong
    • 3
  • Wang Yong-ping
    • 1
  1. 1.Experiment Marine Biology Laboratory, Institute of OceanologyChinese Academy of SciencesQingdaoChina
  2. 2.Haskin Shellfish Research LaboratoryRutgers UniversityPort NorrisUSA
  3. 3.Fisheries CollegeOcean University of QingdaoQingdaoChina

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