Active changes of lignification-related enzymes in pepper response toGlomus intraradices and/orPhytophthora capsici

  • Zheng Hu-zhe
  • Cui Chun-lan
  • Zhang Yu-ting
  • Wang Dan
  • Jing Yu
  • Kim Kil Yong
Plant & Animal Sciences and Biotechnology


The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus ofGlomus intraradices and/or infection with pathogenic strain ofPhytophthora capsici, and the biological control effect ofG. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated withG. intraradices (Gi), (2) plants pre-inoculated withG. intraradices and then infected withP. capsici (Gi+Pc). (3) plants infected withP. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection ofP. capsici was completely eliminated by pre-inoculation with antagonisticG. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45000, 53000 and 114000) were present in Pc-treated roots, while two major bands (53000 and 114000) and one minor band (45000) were present in spectra of Gi+Pc-treated roots, the 45000 POD isozyme was significantly suppressed byG. intraradices, suggesting that the 45000 POD isozyme was induced by the pathogen infection but not induced by the antagonisticG. intraradices. A 60000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonisticG. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected byP. capsici. The results suggested thatG. intraradices is a potentially effective protection agent againstP. capsici.

Key words

Arbuscular mycorrhizal (AM) fungus Glomus intraradices Phytophthora capsici Peroxidase (POD) Polyphenol oxidase (PPO) Phenylalanine ammonia-lyase (PAL) 

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Copyright information

© Zhejiang University Press 2005

Authors and Affiliations

  • Zheng Hu-zhe
    • 1
    • 2
  • Cui Chun-lan
    • 2
  • Zhang Yu-ting
    • 2
  • Wang Dan
    • 3
  • Jing Yu
    • 2
  • Kim Kil Yong
    • 1
  1. 1.Division of Biological and Environmental Chemistry, School of Agriculture and Life ScienceChonnam National UniversityGwangjuKorea
  2. 2.Department of EngineeringLiaoning Agricultural Vocation-Technical CollegeYingkouChina
  3. 3.Department of ForestryShenyang Agricultural UniversityShenyangChina

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