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Journal of Zhejiang University-SCIENCE A

, Volume 2, Issue 1, pp 94–99 | Cite as

Microsatellite alteration and its characteristics in colorectal carcinoma

  • Lai Mao-de
  • Zhang Yu-wei
  • Gao Yu-tong
  • He Chao
Medicine
  • 28 Downloads

Abstract

Objective: To determine the role of microsatellite alterations in carcinogenesis of colorectal carcinoma (CRC). Methods: Alterations of 10 microsatellite loci from 5 different chromosomes were detected in 92 colorectal cancers and their paired normal mucosa by PCR, denatured polyacrylamide gel electrophoresis and silver staining. Associations of microsatellite alterations with clinopathologic parameters were statistically clarifield. Results: Alterations of microsatellite were classified into microsatellite instability type I, type II and loss of heterozygosity (LOH). The carcinoma with ≧30% loci microsatellite alterations was defined as replication error(RER) positive tumors. Of 92 cases, 14 were RER+. Microsatellite alterations of P53(1) and D18S363 loci (64.29%) was most commonly identified in the RER+ tumors. RER+ were more commonly seen in poorly differentiated carcinomas and tended to occur in mucoid carcinomas. The type of microsatellite alterations varied in different histological types of CRC. Conclusions: Microsatellite alteration is a common molecular event in CRC. Different microsatellite loci showed various biologic significance. P53(1) and D18S363 should be essentially detected loci that can show the RER status of tumors.

Key words

microsatellite instability replication error colorectal carcinoma 

Document code

CLC number

R735.34 

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References

  1. Aaltonen, L. A., Deltomaki, P., Leach, F. S., et al., 1993. Clues to pathogenesis of familial colorectal cancer.Science,260:812–816.CrossRefGoogle Scholar
  2. Arzimanoglou, I. I., Gilbert, F., Barber, H. R. K., 1998. Microsatellite instability in human solid tumor.Cancer,82:1808–1819.CrossRefGoogle Scholar
  3. Cawkwell, L., Li, D., Lewis, D., et al., 1995. Microsatellite instability in colorectal cancer: improved assessment using fluorescent polymerase chain reaction.Gastroenterology,109:465–471.CrossRefGoogle Scholar
  4. Dietmaier, W., Wallinger, S., Backer, S., et al., 1997. Diagnostic microsatellite instability: definition and correlation with mismatch repair expression.Cancer Res.,57:4749–4756.Google Scholar
  5. Fan, J., Deveas, S., Zheng, W., et al., 1993. Cancer incidence trends in urban Shanghai.Int J Cancer,53: 764–770.CrossRefGoogle Scholar
  6. Gao, Y., Lai, M., 1998. Detection of microsatellite instability using silver staining technique.Chin J Clin Exp Pathol.,14:193–194 (in Chinese).Google Scholar
  7. Lai, M., 1997. The progress of molecular biology of colorectal cancer.Chin J Clin Exp Pathol. 13:59–60 (in Chinese).Google Scholar
  8. Thibodeau, S. N., French, A. J., Cunningham, J. M., et al., 1998. Microsatellite instability in colorectal cancer: different mutator phenotypes and the principal involvement of hMLH1.Cancer Res.,58:1713–1718.Google Scholar
  9. Xu, N., Qiu, H., Ding, Y., 1998. The clinical pathology features of RER+ colorectal cancer.Chin J Pathol,27:359–361 (in Chinese).Google Scholar
  10. Zhang, Y., Lai, M., 1999. Analyse of false positive results of microsatellite DNA-PCR-silver staining detection.Chin J Pathol.,28:53–54 (in Chinese).Google Scholar

Copyright information

© Zhejiang University Press 2001

Authors and Affiliations

  • Lai Mao-de
    • 1
  • Zhang Yu-wei
    • 1
  • Gao Yu-tong
    • 1
  • He Chao
    • 1
  1. 1.Department of Pathology, Medical CollegeZhejiang UniversityHangzhouChina

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