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Journal of Zhejiang University Science B

, Volume 6, Issue 9, pp 907–912 | Cite as

Effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock

  • Lu Yuan-qiang
  • Cai Xiu-jun
  • Gu Lin-hui
  • Fan Yu-jing
  • Wang Qi
  • Bao De-guo
Article
  • 22 Downloads

Abstract

Objective: To observe the effects of three fluid resuscitation methods on apoptosis of visceral organs in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock and active bleeding was established in 32 SD (Sprague-Dawley) rats. The rats were randomly divided into control group, no fluid resuscitation group (NF group), controlled fluid resuscitation group (NS40 group) and rapid large scale fluid resuscitation group (NS80 group). Each group contained 8 rats. The curative effects were compared. At the same time, the apoptosis in liver, kidney, lung and small intestinal mucosa of survivors after hemorrhage and resuscitation was detected by light microscopy in HE (hematoxylin and eosin) stained tissue sections, flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Results: The survival rate of early fluid resuscitation (14/16) was markedly higher than that of NF group (3/8). There was some apoptosis in liver, kidney, lung and small intestinal mucosa of all survivors. Compared with NF and NS40 groups, the apoptosis of liver, kidney and small intestinal mucosa of NS80 group was obviously increased. Conclusions: Among three fluid resuscitation methods, controlled fluid resuscitation can obviously improve the early survival rate and the apoptosis of liver, kidney and small intestinal mucosa in rats with severe and uncontrolled hemorrhagic shock, and may benefit improvement of prognosis.

Key words

Shock Hemorrhagic Resuscitation Apoptosis 

Document code

CLC number

R605.971 

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References

  1. Angele, M.K., Xu, Y.X., Ayala, A., Schwacha, M.G., Catania, R.K., Cioffi, W.G., Bland, K.I., Chaudry, I.H., 1999. Gender dimorphism in trauma-hemorrhage-induced thymocyte apoptosis.Shock,12: 316–322.PubMedCrossRefGoogle Scholar
  2. Bickell, W.H., Wall, M.J., Jr, Pepe, P.E., Martin, R.R., Ginger, V.F., Allen, M.K., Mattox, K.L., 1994. Immediate versus delayed fluid resuscitation for hypotensive patients with penetrating torso injuries.N. Engl. J. Med.,331: 1105–1109.PubMedCrossRefGoogle Scholar
  3. Burris, D., Rhee, P., Kaufmann, C., Pikoulis, E., Austin, B., Eror, A., DeBraux, S., Guzzi, L., Leppaniemi, A., 1999. Controlled resuscitation for uncontrolled hemorrhagic shock.J. Trauma,46: 216–223.PubMedCrossRefGoogle Scholar
  4. Capone, A.C., Safar, P., Stezoski, W., Tisherman, S., Peitzman, A.B., 1995. Improved outcome with fluid restriction in treatment of uncontrolled hemorrhagic shock.J. Am. Coll. Surg.,180: 49–56.PubMedGoogle Scholar
  5. Chen, X.X., Ye, G.S., Shuai, X.J., Zhu, J., Li, D.P., Yue, C.H., 2004. Healing effect of limited fluid resuscitation treatments on severe thoracic trauma accompanied with hemorrhagic traumatic shock.Chin. J. Crit. Care. Med.,24: 178–180.Google Scholar
  6. Guan, J., Jin, D.D., Jin, L.J., 1998. Apoptosis in multiple organs of rats in early stage of polytrauma combined with shock.Natl. Med. J. China,78: 741–745 (in Chinese).Google Scholar
  7. Kim, S.H., Stezoski, S.W., Safar, P., Capone, A., Tisherman, S., 1997. Hypothermia and minimal fluid resuscitation increase survival after uncontrolled hemorrhagic shock in rats.J. Trauma.,42: 213–222.PubMedCrossRefGoogle Scholar
  8. Meldrum, D.R., Shenkar, R., Sheridan, B.C., Cain, B.S., Abraham, E., Harken, A.H., 1997. Hemorrhage activates myocardial NF-kappaB and increases TNF-alpha in the heart.J. Mol. Cell Cardiol.,29: 2849–2854.PubMedCrossRefGoogle Scholar
  9. Revell, M., Greaves, I., Porter, K., 2003. Endpoints for fluid resuscitation in hemorrhagic shock.J. Trauma.,54: 63–67.Google Scholar
  10. Xu, Y.X., Ayala, A., Monfils, B., Cioffi, W.G., Chaudry, I.H., 1997. Mechanism of intestinal mucosal immune dysfunction following trauma-hemorrhage: Increased apoptosis associated with elevated Fas expression in Peyer's patches.J. Surg. Res.,70: 55–60.PubMedCrossRefGoogle Scholar
  11. Yu, Z.Y., Ono, S., Spatz, M., McCarron, R.M., 2002. Effect of hemorrhagic shock on apoptosis and energy-dependent efflux system in the brain.Neurochem. Res.,27: 1625–1632.PubMedCrossRefGoogle Scholar
  12. Zhang, S., Chen, R.K., Lin, M., He, X.M., 2004. Dynamic change of apoptosis of alveolar cells in ischemia-reperfusion induced pulmonary: An experimental study with rats.Natl. Med. J. China,84: 1597–1600 (in Chinese).Google Scholar

Copyright information

© Zhejiang University Press 2005

Authors and Affiliations

  • Lu Yuan-qiang
    • 1
  • Cai Xiu-jun
    • 1
  • Gu Lin-hui
    • 2
  • Fan Yu-jing
    • 1
  • Wang Qi
    • 1
  • Bao De-guo
    • 1
  1. 1.Department of Emergency, Sir Run Run Shaw Hospital, School of MedicineZhejiang UniversityHangzhouChina
  2. 2.Cancer InstituteZhejiang Tumor HospitalHangzhouChina

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