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Journal of Zhejiang University-SCIENCE A

, Volume 5, Issue 6, pp 743–748 | Cite as

Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract

  • Xu Wen
  • Yao Ke
  • Sun Zhao-hui
  • Wang Kai-jun
  • Shentu Xing-chao
Biomedical Science
  • 55 Downloads

Abstract

Objective: To study the role of calpain in the mechanism of oxidative cataract through detecting the level of intracellular free Ca2+, the expression and proteolytic activity of calpain in the lens epithelial cells (LECs) of H2O2-induced cataract. Methods: Rat lenses were cultured in vitro and cataract was induced by H2O2. The level of intracellular free Ca2+ was measured by fluorescence determination with fura-2/AM. The expression of m-calpain protein in LECs was detected with immunohistochemical method. The proteolytic activity in LECs was measured using a fluorogenic synthetic substrate. Results: There were significant differences of the level of intracellular free Ca2+ (P=0.001, 0.000, 0.000), the expression of m-calpain (P=0.001, 0.000, 0.000) and the proteolytic activity of calpain (P=0.001, 0.000, 0.000) between H2O2-induced and control group at 6, 12 and 24 h, respectively. Conclusions: H2O2 can increase intracellular free Ca2+, then enhance the expression and proteolytic activity of calpain which may play a role in the mechanism of oxidative cataract of rat.

Key words

Cataract Lens epithelial cell Hydrogen peroxide Calpain Calcium 

Document code

CLC number

R776 

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Copyright information

© Zhejiang University Press 2004

Authors and Affiliations

  • Xu Wen
    • 1
  • Yao Ke
    • 1
  • Sun Zhao-hui
    • 1
  • Wang Kai-jun
    • 1
  • Shentu Xing-chao
    • 1
  1. 1.Eye Center, Second Affiliated Hospital, Medical CollegeZhejiang UniversityHangzhouChina

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