Journal of Zhejiang University-SCIENCE A

, Volume 5, Issue 6, pp 689–695 | Cite as

Improved production of spiramycin by mutantStreptomyces ambofaciens

  • Jin Zhi-hua
  • Cen Pei-lin
Material & Chemical Science


Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant,Streptomyces ambofaciens XC 2–37, produced 9% more spiramycin than the parent strainS. ambofaciens XC 1–29. The effects of soybean oil and propyl alcohol on spiramycin production withS. ambofaciens XC 2–37 were studied. The potency ofS. ambofaciens XC 2–37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at 20 hours after incubation in fermentor fermentation. The new process withS. ambofaciens XC 2–37 was scaled up for industrial scale production of spiramycin in a 60 m3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of fermentation were improved by 42.9%.

Key words

Spiramycin Streptomyces ambofaciens Strain improvement Fermentation 

Document code

CLC number



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Copyright information

© Zhejiang University Press 2004

Authors and Affiliations

  • Jin Zhi-hua
    • 1
    • 2
  • Cen Pei-lin
    • 2
  1. 1.Department of Biological and Pharmaceutical Engineering, Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  2. 2.Institute of Bioengineering, College of Materials Science and Chemical EngineeringZhejiang UniversityHangzhouChina

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