The microstructure and gelling mechanism of thermo-responsive chitosan hydrogel system



Thermo-responsive chitosan hydrogel system (TRCHS) was prepared and its microstructure was investigated by scaning electron microscope (SEM) and mercury intrusion poremaster (MIP). Based on analyzing the data, a special porosity property was reported at the first time. Its gelling mechanism was studied by a group of contrast experiments. Results may provide experimental and theoretical supports for how to apply it on tissue engineering scaffold and how to influence or control its essential properties.

Key words

thermo-responsive chitosan hydrogel system (TRCHS) microstructure gelling mechanism 


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  1. [1]
    Chenite A, Chaput C, Wang D,et al. Novel Injectable Neutral Solutions of Chitosan form Biodegradable Gels in Situ[J].Biomaterials, 2000, 21(21):2155CrossRefGoogle Scholar
  2. [2]
    Lee K Y, Ha W S, Park W H. Blood Compatibility and Biodegradability of Partially N-acylated Chitosan Derivatives[J].Biochematerials, 1995, 16(16):1211–6CrossRefGoogle Scholar
  3. [3]
    Molinaro G, Leroux J C, Damas J,et al. Biocompatibility of Thermosensitive Chitosan-based Hydrogels: An in vivo Experimental Approach to Injectable Biomaterials [J].Biomaterials, 2002, 23(13):2717CrossRefGoogle Scholar
  4. [4]
    Tomihata K, Ikada Y. In Vitro and in vivo Degradation of Films of Chitin and Its Deacetylated Derivatives [J].Biomaterials, 1997, 18(7):567CrossRefGoogle Scholar
  5. [5]
    Varum, Kjell M., Myhr,et al. In Vitro Degradation Rates of Partially N-acetylated Chitosans in Human Serum [J].Carbohydrate Research, 1996, 299(2):99CrossRefGoogle Scholar
  6. [6]
    Lahiji A, Sohrabi A, Hungerford DS,et al. Chitosan Supports the Expression of Extracellular Matrix Proteins in Human Osteoblasts and Chondrocytes[J].J. Biomed. Mater. Res., 2000, 51 (4):586–95CrossRefGoogle Scholar
  7. [7]
    Park Y J, Lee Y M, Lee J Y,et al. Controlled Release of Plateletderived Growth Factor-BB from Chondroitin Sulfate-chitosan Sponge for Guided Bone Regeneration [J].J. Control. Release., 2000, 67(2–3):385–394CrossRefGoogle Scholar
  8. [8]
    Park Y J, Lee Y M, Park S N,et al. Platelet Derived Growth Factor Releasing Chitosan Sponge for Periodontal Bone Regeneration[J].Biomaterials, 2000, 21(2):53–59CrossRefGoogle Scholar
  9. [9]
    Lee Y M, Park Y J, Lee S J,et al. The Bone Regenerative Effect of Platelet-derived Growth Factor-BB Delivered with a Chitosan/Tricalcium Phosphate Sponge Carrier[J].J. Periodontal., 2000, 71(3):418–424CrossRefGoogle Scholar
  10. [10]
    Gekko K, Mugishima H, Koga S. Effects of Sugars and Polyols on the Sol-gel Transition of k-carrageenan: Calorimetric Study [J].Int. J. Biol. Macromol., 1987, 9:146CrossRefGoogle Scholar
  11. [11]
    Yao K D, Liu J. Water in Polymers [J].Polymer Science and Engineering, 1999, 15:5Google Scholar

Copyright information

© Editorial Office of Journal of Wuhan University of Technology-Materials Science Edition 2005

Authors and Affiliations

  • Fan Donghui
    • 1
  • Zhang Weiying
    • 1
  • Gu Qisheng
    • 2
  • Xu Zheng
    • 1
  1. 1.School of Material Science and TechnologyTongji UniversityShanghaiChina
  2. 2.Qisheng Institute of Biomaterial TechnologyShanghaiChina

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