Expression of bax in lung cancer cell apoptosis induced by peroxisome proliferator-activated receptor-γ agonists
- 40 Downloads
To discuss the relationship between Bax expression level and lung cancer cell apoptosis induced by peroxisome proliferator-activated receptor-γ (PPAR-γ) agonists.
RT-PCR and Western blot analysis were used to detect PPAR-γ expression in the lung cancer cells, and TUNEL was used to detect apoptosis induced by PPAR-γ agonists, while in situ hybridization and immunohistochemistry were used to monitor the changes of Bax mRNA and protein expression levels after apoptosis induced.
PPAR-γ expression was detectable in two kinds of lung cancer cells (including Non-small cell lung cancer and small cell lung cancer), and PPAR-γ agonists could inhibit lung cancer growth through inducing apoptosis. The apoptostic rates in control group, 15d-PGJ2 group and ciglitazone group were (1.86±0.49)%, (25.8±2.9)%, and (17.3±1.9)% (P<0.01) respectively; Bax mRNA expression rates in the three groups were (8.75±1.36)%, (66.2±12.86)%, and (29.5±6.5)% (P<0.01) respectively; Bax protein expression rates in the three groups were (9.2±1.45)%, (63±10.4)%, and (34.5±6.0)% (P<0.05) respectively.
PPAR-γ is predicted to be a new target in treating lung cancer in the future, and Bax is most likely to work in treating lung cancer apoptosis induced by PPAR-γ agonists as a factor to induced apoptosis.
Key wordsBax PPAR-γ agonists lung cancer cell apoptosis
Unable to display preview. Download preview PDF.
- 2.Wood I. Pro-inflammatory mechanisms of a nonsteroidal anti-inflammatory drug. Trends Endocrinol Metab, 2002, 13: 53.Google Scholar
- 9.Carlos GF, Simone WS, Godefridus JP,et al. Chemotherapy triggers apoptosis in a caspase-8-dependent and mitochondria controlled manner in the non-small cell lung cancer cell line NCI-H460. Cancer Res, 2000, 60: 7133–7141.Google Scholar