Folia Microbiologica

, Volume 37, Issue 2, pp 82–86 | Cite as

Large-scale isolation and partial characterization of plasmid DNA fromB. larvae

  • J. Bodorová-Urgošíková
  • O. Benada
  • P. Tichý


Large-scale preparation of plasmid DNA from twoBacillus larvae strains 423 and 728, honey-bee pathogens, is described. The isolated plasmid DNAs were analyzed by restriction enzyme mapping. No difference in the resulting maps was found for six restriction enzymes. The plasmid DNAs were also compared by Southern blot hybridization and by electron microscopy. The results confirmed the identity of these plasmid DNAs. All these data suggest thatB. larvae strains harbor the same plasmid.


Southern Blot Hybridization Plasmid DNAs Solid Agar Medium Restriction Enzyme Mapping Cellophane Disc 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bakhiet N., Stahly D.P.: Studies on transfection and transformation of protoplasts ofBacillus larvae, Bacillus subtilis, andBacillus popilliae.Appl. Environ. Microbiol. 49, 577–581 (1985).PubMedGoogle Scholar
  2. Bambrick J.F.: Resistance to American foul brood in honey bees.J. Invertebr. Pathol. 9, 30–34 (1967).CrossRefGoogle Scholar
  3. Benada O., Drobníková V., Kalachová, L., Ludvík J.: Plasmid DNA inBacillus larvae J. Apicult. Res. 27, 35–39 (1988).Google Scholar
  4. Davis R.W., Simon M., Davidson N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids, pp. 413–428 in L. Grossman, K. Moldave (Eds.):Methods in Enzymology, Vol. XXI D. Academic Press, New York 1971.Google Scholar
  5. Gordon R.N., Haynes W.C., Paug C.H.N.: The genusBacillus. Handbook U.S. Dept. Agric. No. 427 (1973).Google Scholar
  6. Julian C.S.T., Bulla I.A. Jr.: Physiology of sporeforming bacteria associated with insects. IV. Glucose metabolism inBacillus larvae.J. Bacteriol. 108, 828–834 (1971).PubMedGoogle Scholar
  7. Kleinschmid A.K.: Monolayer techniques in electron microscopy of nucleic acid molecules, pp. 361–377 in I. Grossman, K. Moldave (Eds.):Methods in Enzymology, Vol. XII B. Academic Press, New York 1968.Google Scholar
  8. Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory, New York 1982.Google Scholar
  9. Primrose S.B., Ehrlich S.D.: Isolation of plasmid deletion mutants and study of their instability.Plasmid 6, 193–201 (1981).PubMedCrossRefGoogle Scholar

Copyright information

© Folia Microbiologica 1992

Authors and Affiliations

  • J. Bodorová-Urgošíková
    • 1
  • O. Benada
    • 2
  • P. Tichý
    • 2
  1. 1.Faculty of ScienceCharles UniversityPrague 2Czechoslovakia
  2. 2.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4Czechoslovakia

Personalised recommendations