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Chinese Journal of Integrative Medicine

, Volume 10, Issue 4, pp 289–295 | Cite as

Effect of Danshao Huaxian capsule on the expression of collagen I, III, and caspase-3 in hepatic fibrosis rats

  • Geng Xiao-xia
  • Yang Qin
  • Xie Ru-jia
  • Luo Xin-hua
  • Li Cheng-xiu
  • Cheng Ming-liang
Experimental Work and Research
  • 26 Downloads

Abstract

Objective: To study the effect of Danshao Huaxian capsule (DSHX), a traditional Chinese medical prescription, on the expression of collagen (Col) I, III and cysteinyl aspartate specific proteases-3 (caspase-3) in CCl4-induced hepatic fibrosis in rats. And also it is to explore the mechanism of DSHX in anti-fibrosis.Methods: Eighty male Wistar rats were randomly divided into the normal control group (A), the model group (B), the un-treated model group (C), the low-dose-DSHX treated group (D) and the high-dose-DSHX treated group (E). Except those in Group A, all the other rats were made into hepatic fibrotic models by comprehensive processes including subcutaneous injecting of CCl4, and feeding them with alcoholic high-fat and low-protein diet for 8 weeks. Then the two DSHX-treated groups were treated respectively with low dose (0.5 g/kg) and high dose (1.0 g/kg) DSHX capsule by gastrogavage everyday for 8 weeks. At the end of the experiment, the liver index, levels of hyaluronic acid (HA) and alanine aminotransferase (ALT) in serum, degree of hepatic fibrosis, and urinary excretion of hydroxyproline (Hyp) were measured, and the expression of Col I, Col III and caspase-3 in liver tissues were detected respectively by immunohistochemistric technique.Results: Compared with those in Group B and C, the two DSHX treated groups showed that the liver index, levels of serum HA and ALT and severity of hepatic fibrosis were all significantly lower, the urinary excretion of Hyp was significantly higher; the Col I and Col-III expression was lower (Col I: 1.23±1.14, 1.07 ±0.96 vs 4.18±2.26, 3.22±1.44,P<0.01;Col III: 1.31±0.69, 1.09±0.58 vs 3.04±0.62, 2.23±0.58,P<0.05). At the same time, the expression of caspase-3 in Group E was fewer than Group B and C in hepatocytes (3.09±0.65 vs 9.60±2.32, 8.82±1.45,P<0.01), but it was extensively expressed in fibrous septal cells(4.52±0.87 vs 1.69±0. 23, 2. 98±0. 36,P<0.01).Conclusion: DSHX capsule shows certain therapeutic effect on hepatic fibrosis in rats, and the mechanism might be related with reducing Col I and Col III deposition, inhibiting hepatocyte apoptosis and promoting fibrous septal cells (mainly the activated hepatic stellate cells) apoptosis.

Key words

liver fibrosis collagen cysteinyl aspartate specific proteases-3 apoptosis traditional Chinese herbs 

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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press 2004

Authors and Affiliations

  • Geng Xiao-xia
    • 1
  • Yang Qin
    • 2
  • Xie Ru-jia
    • 2
  • Luo Xin-hua
    • 3
  • Li Cheng-xiu
    • 4
  • Cheng Ming-liang
    • 1
  1. 1.Department of Infectious DiseasesAffiliated Hospital of Guiyang Medical CollegeGuizhou
  2. 2.Department of PathophisiologyGuiyang Medical CollegeChina
  3. 3.Department of Infectious DiseasesPeople’s Hospital of Guizhou ProvinceChina
  4. 4.Department of PharmacologyGuiyang Medical CollegeChina

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