Skip to main content
SpringerLink
Log in

Effect of propyl gallate on activity of cyclooxygenase 1 and 2 in mice’s peritoneal macrophages

  • Experimental Work and Research
  • Published:
Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages.Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F1α(6-keto-PGF1α) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term.Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF1α synthesis at the concentrations of 1 × 10-6, 5 × 10-6 mol/L (P>0.05), but enhanced 6-keto-PGF1α synthesis at the concentrations of 1 × 10-6, 5 × 10-7, 1 × 10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1 × 10-6, 1 × 10-6 mol/L (P< 0.05).Conclusion: Within the range of 1 × 10-5 to 1 × 10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Liu J, Lu EW, Li XG, et al. Effect of propyl gallate on thromboxane B2, arachidonic acid metabolism and platelet aggregation in patients with coronary heart disease or cerebral thrombosis. ChinMedJ 1983;63(8): 477–481.

    CAS  Google Scholar 

  2. Chen XX, He B. Protection of propyl gallate against lipid peroxdation induced by cerebral ischemia-reperfusion in rats. Chin J Mod Applied Pharmacy 2000;17(4): 261–264.

    Google Scholar 

  3. Van Wauwe J, Goossens J. Effects of antioxidants on cyclooxygenase and lipoxygenase activities in intact human platelets: comparison with indomethacin and ETYA. Prostaglandins 1983; 26(5): 725–730.

    Article  PubMed  Google Scholar 

  4. Hu YF, Cheng GF. Establishment of screening models for selective cyclooxygenase-2 inhibitors. Acta Pharm Sin 2001; 35: 343–346.

    Google Scholar 

  5. Ruitenberg JJ, Waters CA. A rapid flow cytometric method for the detection of intracellular cyclooxygenases in human whole blood monocytes and a COX-2 inducible human cell line. J Immunol Meth 2003;274: 93–104.

    Article  CAS  Google Scholar 

  6. Smith WL, Garavito RM, DeWitt DL. Prostaglandin endoperoxide H synthases (cyclooxygenase)-l and -2. J Biol Chem 1996;271: 33157–33160.

    Article  PubMed  CAS  Google Scholar 

  7. Williams CS, Du Bois RN. Prostaglandin endoperoxide synthase: Why two isoforms? Am J Physiol 1996; 270 (3ptl): 393.

    Google Scholar 

  8. Mitchell JA, Larkins S, Williams TJ. Cyclooxygenase-2: Regulation and relevance in inflammation. Biochem Pharmacol 1995; 50(10): 1535–1542.

    Article  PubMed  CAS  Google Scholar 

  9. Giuliano F, Warner TD. Ex vivo assay to determine the cyclooxygenase selectivity of nonsteroidal anti-inflammatory drugs. Br J Pharmacol 1999;126: 1824–1839.

    Article  PubMed  CAS  Google Scholar 

  10. Shen F, Bai AP, Guo ZR, et al. Inhibitory effect of 3,4-diary-3-pyrrolin-2-one derivatives on cyclooxygenase 1 and 2 in murine peritoneal macrophages. Acta Pharmacol Sin 2002;23: 762–768.

    PubMed  CAS  Google Scholar 

  11. Patronp C. Aspirin as an antiplatelet drug. N Eng J Med 1994; 330: 1287–1294.

    Article  Google Scholar 

  12. Panganamala RV, Miller JS, Gwebu ET, et al. Differential inhibitory effects of vitamin E and other antioxidants on prostaglandin synthetase, platelet aggregation and lipoxidase. Prostaglandins 1977;14(2): 261–271.

    Article  PubMed  CAS  Google Scholar 

  13. Xiao H, Kovics R, Jackson V, et al. Effects of platelet inhibitors on propyl gallate-induced platelet aggregation, protein tyrosine phosphorylation, and platelet factor 3 activation. Blood Coagul Fibrinolysis 2004,15(3): 199–206.

    Article  PubMed  CAS  Google Scholar 

  14. Rothwell SW, Fudge JM, Chen WK, et al. Addition of a propyl gallate-based procoagulation to a fibrin bandage improves hemostatic performance in a swine arterial bleeding model. Throm Res 2003;108: 335–340.

    Article  CAS  Google Scholar 

  15. Derek AW, Adrian RM, Paul R, et al. COX-1,COX-2,COX-3 and the future treatment of chronic inflammatory disease. Lancet 2000;355(9204): 646–648.

    Article  Google Scholar 

  16. Franzone JS, Natale T, Cirillo R, et al. Influence of propyl-gallate and 2-mercaptopropionylglycine on the development of acute inflammatory reactions and on biosynthesis of PGE2. Boll Soc Ital Biol Sper 1980;56 (24): 2539–2545.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Xiyuan Hospital, China Academy of Traditional Chinese Medcine, 100091, Beijing, China

    Yin Hui-jun, Jiang Yue-rong & Chen Ke-ji

  2. Fujian Mindong Rejuvenation Pharmaceutical Co., Ltd., Fuzhou

    Wu Xiao-hua

  3. Institute of Materia Medica, Chinese Academy of Medical Sciences, China

    Chen Xiao-hong

Authors
  1. Yin Hui-jun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiang Yue-rong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wu Xiao-hua
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chen Xiao-hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chen Ke-ji
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work is supported by the National Natural Science Foundation (item number: 90209054)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hui-jun, Y., Yue-rong, J., Xiao-hua, W. et al. Effect of propyl gallate on activity of cyclooxygenase 1 and 2 in mice’s peritoneal macrophages. Chin. J. Integr. Med. 10, 213–217 (2004). https://doi.org/10.1007/BF02836411

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02836411

Keywords

Search

Navigation