Chinese Journal of Integrative Medicine

, Volume 10, Issue 3, pp 213–217 | Cite as

Effect of propyl gallate on activity of cyclooxygenase 1 and 2 in mice’s peritoneal macrophages

  • Yin Hui-jun
  • Jiang Yue-rong
  • Wu Xiao-hua
  • Chen Xiao-hong
  • Chen Ke-ji
Experimental Work and Research


Objective: To investigate the effect of Red Peony 801 (propyl gallate,PrG) on cyclooxygenase (COX) activity in murine peritoneal macrophages.Methods: A screening model for COX inhibitors in vitro based on murine peritoneal macrophages was used. COX-1 activity was reflected by the level of 6-ketoprostaglandin F(6-keto-PGF) in supernatants of cultured macrophages which were stimulated with calcium ionophore A23187 for a short-term, while COX-2 activity was reflected by the level of prostaglandin E2 (PGE2) in supernatants of cultured macrophages which were stimulated with lipopolysaccharide (LPS) for a long-term.Results: PrG did not affect A23187-induced, COX-1-derived 6-keto-PGF synthesis at the concentrations of 1 × 10-6, 5 × 10-6 mol/L (P>0.05), but enhanced 6-keto-PGF synthesis at the concentrations of 1 × 10-6, 5 × 10-7, 1 × 10-7 mol/L (P<0.01) in vitro, and showed a good dose-dependent manner. It inhibited LPS-induced, COX-2-derived PGE2 synthesis at the concentrations of 1 × 10-6, 1 × 10-6 mol/L (P< 0.05).Conclusion: Within the range of 1 × 10-5 to 1 × 10-7 mol/L, PrG activated COX-1 at lower concentrations and inhibited COX-2 at higher concentrations in murine peritoneal macrophages.


propyl gallate cyclooxygenase 1 cyclooxygenase 2 inflammation 


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Copyright information

© The Chinese Journal of Integrated Traditional and Western Medicine Press 2004

Authors and Affiliations

  • Yin Hui-jun
    • 1
  • Jiang Yue-rong
    • 1
  • Wu Xiao-hua
    • 2
  • Chen Xiao-hong
    • 3
  • Chen Ke-ji
    • 1
  1. 1.Xiyuan HospitalChina Academy of Traditional Chinese MedcineBeijingChina
  2. 2.Fujian Mindong Rejuvenation Pharmaceutical Co., Ltd.Fuzhou
  3. 3.Institute of Materia MedicaChinese Academy of Medical SciencesChina

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